Selective interferon-induced enhancement of tumor-associated antigens on a spectrum of freshly isolated human adenocarcinoma cells

Fiorella Guadagni, Jeffrey Schlom, William W. Johnston, Cheryl A. Szpak, David Goldstein, Richard Smalley, Jean F. Simpson, Ernest C. Borden, Sidney Pestka, John W. Greiner

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Freshly isolated cells from patients with pleural or peri-toneal effusions cytologically diagnosed as adenocarcinoma(n = 43), malignant nonepithelial neoplasms (n = 10), and benign (n = 8) were analyzed for expression of constitutive levels of the tumor antigens TAG-72 [recognized by mono-clonal antibody (MAb) B72.3] and carcinoembryonic anti-gen (CEA) (recognized by MAb COL-4) as well as the classI and class II major histocompatibility (MHC) antigens, and the ability of human interferons (Hu-IFNs) to enhance cellsurface expression of those antigens as measured by MAb binding. Both type I and type II IFNs enhanced the expres-sion of TAG-72 and CEA and altered the level of expressionof the MHC antigens. Comparative studies of three differ-ent Hu-IFNs (IFN-αA, IFN.-βser, and IFN-λ) revealed that IFN-λ was the most potent in augmenting either B72.3 or COL-4 binding. Unlike the IFN-λ -mediated induction of the class II human leukocyte antigens, the change in tu-mor antigen expression consisted of enhanced constitutiveantigen expression; de novo induction of either TAG-72 orCEA could not be achieved by either type I or type IIIFN. Of 43 effusions isolated from different adenocarcinoma pa-tients, 42 (97.7%) expressed either CEA or TAG-72, and treatment with Hu-IFN increased the level of expression of either antigen in 36 of 42 samples (85.7%). These studies demonstrate the augmentation of tumor-associated antigenson human carcinoma cells isolated from serous effusions by Hu-IFNs which may be used to enhance the targeting of con-jugated MAbs to human carcinoma lesions. [J Natl CancerInst 81:502-512, 1989]

Original languageEnglish
Pages (from-to)502-512
Number of pages11
JournalJournal of the National Cancer Institute
Issue number7
Publication statusPublished - Apr 5 1989

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Oncology
  • Cancer Research


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