Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction

Paolo Fiorina, Gabriele Torriani, Chiara Gremizzi, Alberto M. Davalli, Elena Orsenigo, Marta Bruno Ventre, Giacomo Dell'Antonio, Valerie Di Carlo, Silvano Rossini, Antonio Secchi

Research output: Contribution to journalArticlepeer-review

Abstract

Intra-graft infiltrating cells apoptosis was evaluated in 20 consecutive kidney-pancreas transplanted (KP) patients without kidney rejection. Two fine-needle aspirated biopsy (FNAB) and two peripheral blood lymphocytes (PBL) samples were obtained 14 days after transplantation. Immunosuppression was based on anti-Thymoglobulins (ATG) induction for 7 days and cyclosporine/mofetil mycophenolate as maintenance therapy. Ten matched healthy subjects were chosen as controls for PBL. Lymphocyte phenotypes and activation markers, apoptotic rate and lymphocyte expression of pro/anti-apoptotic molecules were analysed by flow cytometry analysis (FACS). Lymphocyte phenotypes and activation markers: higher levels of CD8 and CD4DR were evident in the graft (p <0.05) than in PBL, CD3CD25 in PBL were higher in transplanted patients than in controls. Apoptotic rate and lymphocyte expression of pro- and anti-apoptotic molecules: a higher expression of annexin V, together with reduced lymphocytes CD95L, iNOs and Bcl-2 expression (PBL = 97.7 ± 1.1% vs FNAB = 81.9 ± 15.1%; p <0.05) were evident in the graft than in PBL. In KP patients intra-graft apoptosis and reduced anti-apoptotic molecules were evident after ATG induction.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalTransplant International
Volume17
Issue number10
DOIs
Publication statusPublished - Oct 2004

Keywords

  • Anti-Thymoglobulin induction
  • Apoptosis
  • bcl-2
  • FNAB

ASJC Scopus subject areas

  • Transplantation

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