Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction

Paolo Fiorina, Gabriele Torriani, Chiara Gremizzi, Alberto M. Davalli, Elena Orsenigo, Marta Bruno Ventre, Giacomo Dell'Antonio, Valerie Di Carlo, Silvano Rossini, Antonio Secchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intra-graft infiltrating cells apoptosis was evaluated in 20 consecutive kidney-pancreas transplanted (KP) patients without kidney rejection. Two fine-needle aspirated biopsy (FNAB) and two peripheral blood lymphocytes (PBL) samples were obtained 14 days after transplantation. Immunosuppression was based on anti-Thymoglobulins (ATG) induction for 7 days and cyclosporine/mofetil mycophenolate as maintenance therapy. Ten matched healthy subjects were chosen as controls for PBL. Lymphocyte phenotypes and activation markers, apoptotic rate and lymphocyte expression of pro/anti-apoptotic molecules were analysed by flow cytometry analysis (FACS). Lymphocyte phenotypes and activation markers: higher levels of CD8 and CD4DR were evident in the graft (p <0.05) than in PBL, CD3CD25 in PBL were higher in transplanted patients than in controls. Apoptotic rate and lymphocyte expression of pro- and anti-apoptotic molecules: a higher expression of annexin V, together with reduced lymphocytes CD95L, iNOs and Bcl-2 expression (PBL = 97.7 ± 1.1% vs FNAB = 81.9 ± 15.1%; p <0.05) were evident in the graft than in PBL. In KP patients intra-graft apoptosis and reduced anti-apoptotic molecules were evident after ATG induction.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalTransplant International
Volume17
Issue number10
DOIs
Publication statusPublished - Oct 2004

Fingerprint

Fas Ligand Protein
Pancreas
Down-Regulation
Lymphocytes
Apoptosis
Transplants
Kidney
Lymphocyte Activation
Fine Needle Biopsy
Mycophenolic Acid
Phenotype
thymoglobulin
Annexin A5
Immunosuppression
Cyclosporine
Healthy Volunteers
Flow Cytometry
Transplantation

Keywords

  • Anti-Thymoglobulin induction
  • Apoptosis
  • bcl-2
  • FNAB

ASJC Scopus subject areas

  • Transplantation

Cite this

Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction. / Fiorina, Paolo; Torriani, Gabriele; Gremizzi, Chiara; Davalli, Alberto M.; Orsenigo, Elena; Bruno Ventre, Marta; Dell'Antonio, Giacomo; Carlo, Valerie Di; Rossini, Silvano; Secchi, Antonio.

In: Transplant International, Vol. 17, No. 10, 10.2004, p. 603-608.

Research output: Contribution to journalArticle

Fiorina, Paolo ; Torriani, Gabriele ; Gremizzi, Chiara ; Davalli, Alberto M. ; Orsenigo, Elena ; Bruno Ventre, Marta ; Dell'Antonio, Giacomo ; Carlo, Valerie Di ; Rossini, Silvano ; Secchi, Antonio. / Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction. In: Transplant International. 2004 ; Vol. 17, No. 10. pp. 603-608.
@article{0a2d775137e44409b95d6a12bdd1ad91,
title = "Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction",
abstract = "Intra-graft infiltrating cells apoptosis was evaluated in 20 consecutive kidney-pancreas transplanted (KP) patients without kidney rejection. Two fine-needle aspirated biopsy (FNAB) and two peripheral blood lymphocytes (PBL) samples were obtained 14 days after transplantation. Immunosuppression was based on anti-Thymoglobulins (ATG) induction for 7 days and cyclosporine/mofetil mycophenolate as maintenance therapy. Ten matched healthy subjects were chosen as controls for PBL. Lymphocyte phenotypes and activation markers, apoptotic rate and lymphocyte expression of pro/anti-apoptotic molecules were analysed by flow cytometry analysis (FACS). Lymphocyte phenotypes and activation markers: higher levels of CD8 and CD4DR were evident in the graft (p <0.05) than in PBL, CD3CD25 in PBL were higher in transplanted patients than in controls. Apoptotic rate and lymphocyte expression of pro- and anti-apoptotic molecules: a higher expression of annexin V, together with reduced lymphocytes CD95L, iNOs and Bcl-2 expression (PBL = 97.7 ± 1.1{\%} vs FNAB = 81.9 ± 15.1{\%}; p <0.05) were evident in the graft than in PBL. In KP patients intra-graft apoptosis and reduced anti-apoptotic molecules were evident after ATG induction.",
keywords = "Anti-Thymoglobulin induction, Apoptosis, bcl-2, FNAB",
author = "Paolo Fiorina and Gabriele Torriani and Chiara Gremizzi and Davalli, {Alberto M.} and Elena Orsenigo and {Bruno Ventre}, Marta and Giacomo Dell'Antonio and Carlo, {Valerie Di} and Silvano Rossini and Antonio Secchi",
year = "2004",
month = "10",
doi = "10.1007/s00147-004-0783-7",
language = "English",
volume = "17",
pages = "603--608",
journal = "Transplant International",
issn = "0934-0874",
publisher = "Blackwell Publishing Ltd",
number = "10",

}

TY - JOUR

T1 - Selective intra-graft apoptosis and down-regulation of lymphocyte bcl-2, iNOs and CD95L expression in kidney-pancreas transplanted patients after anti-Thymoglobulin induction

AU - Fiorina, Paolo

AU - Torriani, Gabriele

AU - Gremizzi, Chiara

AU - Davalli, Alberto M.

AU - Orsenigo, Elena

AU - Bruno Ventre, Marta

AU - Dell'Antonio, Giacomo

AU - Carlo, Valerie Di

AU - Rossini, Silvano

AU - Secchi, Antonio

PY - 2004/10

Y1 - 2004/10

N2 - Intra-graft infiltrating cells apoptosis was evaluated in 20 consecutive kidney-pancreas transplanted (KP) patients without kidney rejection. Two fine-needle aspirated biopsy (FNAB) and two peripheral blood lymphocytes (PBL) samples were obtained 14 days after transplantation. Immunosuppression was based on anti-Thymoglobulins (ATG) induction for 7 days and cyclosporine/mofetil mycophenolate as maintenance therapy. Ten matched healthy subjects were chosen as controls for PBL. Lymphocyte phenotypes and activation markers, apoptotic rate and lymphocyte expression of pro/anti-apoptotic molecules were analysed by flow cytometry analysis (FACS). Lymphocyte phenotypes and activation markers: higher levels of CD8 and CD4DR were evident in the graft (p <0.05) than in PBL, CD3CD25 in PBL were higher in transplanted patients than in controls. Apoptotic rate and lymphocyte expression of pro- and anti-apoptotic molecules: a higher expression of annexin V, together with reduced lymphocytes CD95L, iNOs and Bcl-2 expression (PBL = 97.7 ± 1.1% vs FNAB = 81.9 ± 15.1%; p <0.05) were evident in the graft than in PBL. In KP patients intra-graft apoptosis and reduced anti-apoptotic molecules were evident after ATG induction.

AB - Intra-graft infiltrating cells apoptosis was evaluated in 20 consecutive kidney-pancreas transplanted (KP) patients without kidney rejection. Two fine-needle aspirated biopsy (FNAB) and two peripheral blood lymphocytes (PBL) samples were obtained 14 days after transplantation. Immunosuppression was based on anti-Thymoglobulins (ATG) induction for 7 days and cyclosporine/mofetil mycophenolate as maintenance therapy. Ten matched healthy subjects were chosen as controls for PBL. Lymphocyte phenotypes and activation markers, apoptotic rate and lymphocyte expression of pro/anti-apoptotic molecules were analysed by flow cytometry analysis (FACS). Lymphocyte phenotypes and activation markers: higher levels of CD8 and CD4DR were evident in the graft (p <0.05) than in PBL, CD3CD25 in PBL were higher in transplanted patients than in controls. Apoptotic rate and lymphocyte expression of pro- and anti-apoptotic molecules: a higher expression of annexin V, together with reduced lymphocytes CD95L, iNOs and Bcl-2 expression (PBL = 97.7 ± 1.1% vs FNAB = 81.9 ± 15.1%; p <0.05) were evident in the graft than in PBL. In KP patients intra-graft apoptosis and reduced anti-apoptotic molecules were evident after ATG induction.

KW - Anti-Thymoglobulin induction

KW - Apoptosis

KW - bcl-2

KW - FNAB

UR - http://www.scopus.com/inward/record.url?scp=19944427141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944427141&partnerID=8YFLogxK

U2 - 10.1007/s00147-004-0783-7

DO - 10.1007/s00147-004-0783-7

M3 - Article

VL - 17

SP - 603

EP - 608

JO - Transplant International

JF - Transplant International

SN - 0934-0874

IS - 10

ER -