Selective, on-column extraction of organochlorine pesticide residues from milk

A. Di Muccio, M. Rizzica, A. Ausili, I. Camoni, B. Dommarco, F. Vergori

Research output: Contribution to journalArticle

Abstract

A rapid procedure has been developed that allows a single-step, selective extraction of organochlorine pesticide (OCP) residues from milk on solid-matrix disposable columns by means of acetonitrile-saturated light petroleum. Recovery experiments were carried out on homogenized milk (3.6% fat content) spiked with an ethanolic solution of nine OCPs, viz., HCB, α-HCH, β-HCH, γ-HCH, heptachlor epoxide, dieldrin, endrin, p,p'-DDE and p,p'-DDT, at levels ranging from 0.002 mg/kg for α-HCH to 0.008 mg/kg for p,p'-DDT. Average recoveries of four replicates were 77% for HCB and almost quantitative (94-113%) for the other pesticides. with relative standard deviations from 2.9 to 7.3%. Coextracted fatty material amounted to about 5 mg/ml of milk before the clean-up. The described procedure also showed a satisfactory performance with milk powder. The extraction procedure requires about 60 min. The main advantages are that emulsions do not occur, several samples can be run in parallel by a single operator, reusable glassware is not needed and simple operations are required.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalJournal of Chromatography
Volume456
Issue number1
Publication statusPublished - 1988

Fingerprint

Pesticide Residues
Milk
Hexachlorobenzene
Dichlorodiphenyl Dichloroethylene
DDT
Heptachlor Epoxide
Endrin
Dieldrin
Recovery
Petroleum
Emulsions
Pesticides
Powders
Fats
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Di Muccio, A., Rizzica, M., Ausili, A., Camoni, I., Dommarco, B., & Vergori, F. (1988). Selective, on-column extraction of organochlorine pesticide residues from milk. Journal of Chromatography, 456(1), 143-148.

Selective, on-column extraction of organochlorine pesticide residues from milk. / Di Muccio, A.; Rizzica, M.; Ausili, A.; Camoni, I.; Dommarco, B.; Vergori, F.

In: Journal of Chromatography, Vol. 456, No. 1, 1988, p. 143-148.

Research output: Contribution to journalArticle

Di Muccio, A, Rizzica, M, Ausili, A, Camoni, I, Dommarco, B & Vergori, F 1988, 'Selective, on-column extraction of organochlorine pesticide residues from milk', Journal of Chromatography, vol. 456, no. 1, pp. 143-148.
Di Muccio A, Rizzica M, Ausili A, Camoni I, Dommarco B, Vergori F. Selective, on-column extraction of organochlorine pesticide residues from milk. Journal of Chromatography. 1988;456(1):143-148.
Di Muccio, A. ; Rizzica, M. ; Ausili, A. ; Camoni, I. ; Dommarco, B. ; Vergori, F. / Selective, on-column extraction of organochlorine pesticide residues from milk. In: Journal of Chromatography. 1988 ; Vol. 456, No. 1. pp. 143-148.
@article{d31c25fe83c84162a8fbfdfa7ba7ca05,
title = "Selective, on-column extraction of organochlorine pesticide residues from milk",
abstract = "A rapid procedure has been developed that allows a single-step, selective extraction of organochlorine pesticide (OCP) residues from milk on solid-matrix disposable columns by means of acetonitrile-saturated light petroleum. Recovery experiments were carried out on homogenized milk (3.6{\%} fat content) spiked with an ethanolic solution of nine OCPs, viz., HCB, α-HCH, β-HCH, γ-HCH, heptachlor epoxide, dieldrin, endrin, p,p'-DDE and p,p'-DDT, at levels ranging from 0.002 mg/kg for α-HCH to 0.008 mg/kg for p,p'-DDT. Average recoveries of four replicates were 77{\%} for HCB and almost quantitative (94-113{\%}) for the other pesticides. with relative standard deviations from 2.9 to 7.3{\%}. Coextracted fatty material amounted to about 5 mg/ml of milk before the clean-up. The described procedure also showed a satisfactory performance with milk powder. The extraction procedure requires about 60 min. The main advantages are that emulsions do not occur, several samples can be run in parallel by a single operator, reusable glassware is not needed and simple operations are required.",
author = "{Di Muccio}, A. and M. Rizzica and A. Ausili and I. Camoni and B. Dommarco and F. Vergori",
year = "1988",
language = "English",
volume = "456",
pages = "143--148",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Selective, on-column extraction of organochlorine pesticide residues from milk

AU - Di Muccio, A.

AU - Rizzica, M.

AU - Ausili, A.

AU - Camoni, I.

AU - Dommarco, B.

AU - Vergori, F.

PY - 1988

Y1 - 1988

N2 - A rapid procedure has been developed that allows a single-step, selective extraction of organochlorine pesticide (OCP) residues from milk on solid-matrix disposable columns by means of acetonitrile-saturated light petroleum. Recovery experiments were carried out on homogenized milk (3.6% fat content) spiked with an ethanolic solution of nine OCPs, viz., HCB, α-HCH, β-HCH, γ-HCH, heptachlor epoxide, dieldrin, endrin, p,p'-DDE and p,p'-DDT, at levels ranging from 0.002 mg/kg for α-HCH to 0.008 mg/kg for p,p'-DDT. Average recoveries of four replicates were 77% for HCB and almost quantitative (94-113%) for the other pesticides. with relative standard deviations from 2.9 to 7.3%. Coextracted fatty material amounted to about 5 mg/ml of milk before the clean-up. The described procedure also showed a satisfactory performance with milk powder. The extraction procedure requires about 60 min. The main advantages are that emulsions do not occur, several samples can be run in parallel by a single operator, reusable glassware is not needed and simple operations are required.

AB - A rapid procedure has been developed that allows a single-step, selective extraction of organochlorine pesticide (OCP) residues from milk on solid-matrix disposable columns by means of acetonitrile-saturated light petroleum. Recovery experiments were carried out on homogenized milk (3.6% fat content) spiked with an ethanolic solution of nine OCPs, viz., HCB, α-HCH, β-HCH, γ-HCH, heptachlor epoxide, dieldrin, endrin, p,p'-DDE and p,p'-DDT, at levels ranging from 0.002 mg/kg for α-HCH to 0.008 mg/kg for p,p'-DDT. Average recoveries of four replicates were 77% for HCB and almost quantitative (94-113%) for the other pesticides. with relative standard deviations from 2.9 to 7.3%. Coextracted fatty material amounted to about 5 mg/ml of milk before the clean-up. The described procedure also showed a satisfactory performance with milk powder. The extraction procedure requires about 60 min. The main advantages are that emulsions do not occur, several samples can be run in parallel by a single operator, reusable glassware is not needed and simple operations are required.

UR - http://www.scopus.com/inward/record.url?scp=0024267336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024267336&partnerID=8YFLogxK

M3 - Article

VL - 456

SP - 143

EP - 148

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1

ER -