Selective, on-column extraction of organochlorine pesticide residues from milk

Alfonso Di Muccio, Marina Rizzica, Antonella Ausili, Ivano Camoni, Roberto Dommarco, Fabio Vergori

Research output: Contribution to journalArticle

Abstract

A rapid procedure has been developed that allows a single-step, selective extraction of organochlorine pesticide (OCP) residues from milk on solid-matrix disposable columns by means of acetonitrile-saturated light petroleum. Recovery experiments were carried out on homogenized milk (3.6% fat content) spiked with an ethanolic solution of nine OCPs, viz., HCB, α-HCH, β-HCH, γ-HCH, heptachlor epoxide, dieldrin, endrin, p,p′-DDE and p,p′-DDT, at levels ranging from 0.002 mg/kg for α-HCH to 0.008 mg/kg for p,p′-DDT. Average recoveries of four replicates were 77% for HCB and almost quantitative (94-113%) for the other pesticides, with relative standard deviations from 2.9 to 7.3%. Coextracted fatty material amounted to about 5 mg/ml of milk before the clean-up. The described procedure also showed a satisfactory performance with milk powder. The extraction procedure requires about 60 min. The main advantages are that emulsions do not occur, several samples can be run in parallel by a single operator, reusable glassware is not needed and simple operations are required.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalJournal of Chromatography A
Volume456
Issue numberC
DOIs
Publication statusPublished - May 30 1986

ASJC Scopus subject areas

  • Analytical Chemistry

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