TY - JOUR
T1 - Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses
AU - Granucci, Francesca
AU - Di Tota, Francesco P.
AU - Raimondi, Giorgio
AU - Citterio, Stefania
AU - Rescigno, Maria
AU - Ricciardi-Castagnoli, Paola
PY - 2001
Y1 - 2001
N2 - Co-stimulation provided by the B7 family of proteins underpins the development of protective immunity. There are three identified members of this family: CD80, its splice variant IgV-CD80 and CD86. It has hitherto been difficult to analyze the expression and function of IgV-CD80 since there are no appropriate reagents capable of distinguishing it from CD80. We have generated mice, by gene targeting, the lack CD80 whilst maintaining expression of IgV-CD80. Mutant animals did not delete T cells bearing mammary tumor virus-reactive TCR as efficiently as wild-type animals. We also demonstrate the importance of IgV-CD80 in the responses of recently activated cells and reveal a role for CD80 in sustaining T cell responses. CD86, whilst critical to primary T cell activation, made only a minor contribution to re-activation of normal cells.
AB - Co-stimulation provided by the B7 family of proteins underpins the development of protective immunity. There are three identified members of this family: CD80, its splice variant IgV-CD80 and CD86. It has hitherto been difficult to analyze the expression and function of IgV-CD80 since there are no appropriate reagents capable of distinguishing it from CD80. We have generated mice, by gene targeting, the lack CD80 whilst maintaining expression of IgV-CD80. Mutant animals did not delete T cells bearing mammary tumor virus-reactive TCR as efficiently as wild-type animals. We also demonstrate the importance of IgV-CD80 in the responses of recently activated cells and reveal a role for CD80 in sustaining T cell responses. CD86, whilst critical to primary T cell activation, made only a minor contribution to re-activation of normal cells.
KW - Alternative splicing
KW - CD80
KW - Clonal deletion
KW - Co-stimulation
KW - Gene targeting
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U2 - 10.1002/1521-4141(200101)31:1<118::AID-IMMU118>3.0.CO;2-X
DO - 10.1002/1521-4141(200101)31:1<118::AID-IMMU118>3.0.CO;2-X
M3 - Article
C2 - 11169445
AN - SCOPUS:0035133292
VL - 31
SP - 118
EP - 127
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 1
ER -