Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses

Francesca Granucci, Francesco P. Di Tota, Giorgio Raimondi, Stefania Citterio, Maria Rescigno, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Co-stimulation provided by the B7 family of proteins underpins the development of protective immunity. There are three identified members of this family: CD80, its splice variant IgV-CD80 and CD86. It has hitherto been difficult to analyze the expression and function of IgV-CD80 since there are no appropriate reagents capable of distinguishing it from CD80. We have generated mice, by gene targeting, the lack CD80 whilst maintaining expression of IgV-CD80. Mutant animals did not delete T cells bearing mammary tumor virus-reactive TCR as efficiently as wild-type animals. We also demonstrate the importance of IgV-CD80 in the responses of recently activated cells and reveal a role for CD80 in sustaining T cell responses. CD86, whilst critical to primary T cell activation, made only a minor contribution to re-activation of normal cells.

Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalEuropean Journal of Immunology
Volume31
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Clonal Deletion
T-Lymphocytes
Oncogenic Viruses
Wild Animals
Gene Targeting
Immunity
Breast Neoplasms
Proteins

Keywords

  • Alternative splicing
  • CD80
  • Clonal deletion
  • Co-stimulation
  • Gene targeting

ASJC Scopus subject areas

  • Immunology

Cite this

Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses. / Granucci, Francesca; Di Tota, Francesco P.; Raimondi, Giorgio; Citterio, Stefania; Rescigno, Maria; Ricciardi-Castagnoli, Paola.

In: European Journal of Immunology, Vol. 31, No. 1, 2001, p. 118-127.

Research output: Contribution to journalArticle

Granucci, Francesca ; Di Tota, Francesco P. ; Raimondi, Giorgio ; Citterio, Stefania ; Rescigno, Maria ; Ricciardi-Castagnoli, Paola. / Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses. In: European Journal of Immunology. 2001 ; Vol. 31, No. 1. pp. 118-127.
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AU - Rescigno, Maria

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