Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses

Francesca Granucci, Francesco P. Di Tota, Giorgio Raimondi, Stefania Citterio, Maria Rescigno, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

Abstract

Co-stimulation provided by the B7 family of proteins underpins the development of protective immunity. There are three identified members of this family: CD80, its splice variant IgV-CD80 and CD86. It has hitherto been difficult to analyze the expression and function of IgV-CD80 since there are no appropriate reagents capable of distinguishing it from CD80. We have generated mice, by gene targeting, the lack CD80 whilst maintaining expression of IgV-CD80. Mutant animals did not delete T cells bearing mammary tumor virus-reactive TCR as efficiently as wild-type animals. We also demonstrate the importance of IgV-CD80 in the responses of recently activated cells and reveal a role for CD80 in sustaining T cell responses. CD86, whilst critical to primary T cell activation, made only a minor contribution to re-activation of normal cells.

Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalEuropean Journal of Immunology
Volume31
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Alternative splicing
  • CD80
  • Clonal deletion
  • Co-stimulation
  • Gene targeting

ASJC Scopus subject areas

  • Immunology

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