Selective targeting of proteins by hybrid polyoxometalates: Interaction between a bis-biotinylated hybrid conjugate and avidin

Valeria A. Zamolo, Gloria Modugno, Elisa Lubian, Alessandro Cazzolaro, Fabrizio Mancin, Livia Giotta, Disma Mastrogiacomo, Ludovico Valli, Alessandra Saccani, Silke Krol, Marcella Bonchio, Mauro Carraro

Research output: Contribution to journalArticle

Abstract

The Keggin-type polyoxometalate [γ-SiW10O36]8- was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique were used to highlight the unique interplay of supramolecular interactions between the homotetrameric protein and the bis-functionalized polyanion. In particular, the dual recognition mechanism of the avidin encompasses (i) a complementary electrostatic association between the anionic surface of the polyoxotungstate and each positively charged avidin subunit and (ii) specific host-guest interactions between each biotinylated arm and a corresponding pocket on the tetramer subunits. The assembly exhibits peroxidase-like reactivity and it was used in aqueous solution for L-methionine methyl ester oxidation by H2O2. The recognition phenomenon was then exploited for the preparation of layer-by-layer films, whose structural evolution was monitored in situ by ATR-FTIR spectroscopy. Finally, cell tracking studies were performed by exploiting the specific interactions with a labeled streptavidin.

Original languageEnglish
Article number278
JournalFrontiers in Chemistry
Volume6
Issue numberJUL
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • Avidin
  • Bio-hybrids
  • Biotin
  • Oxidation catalysis
  • Polyoxometalates
  • Recognition
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Chemistry(all)

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  • Cite this

    Zamolo, V. A., Modugno, G., Lubian, E., Cazzolaro, A., Mancin, F., Giotta, L., Mastrogiacomo, D., Valli, L., Saccani, A., Krol, S., Bonchio, M., & Carraro, M. (2018). Selective targeting of proteins by hybrid polyoxometalates: Interaction between a bis-biotinylated hybrid conjugate and avidin. Frontiers in Chemistry, 6(JUL), [278]. https://doi.org/10.3389/fchem.2018.00278