Selective up-regulation of phospholipase C-β2 during granulocytic differentiation of normal and leukemic hematopoietic progenitors

Valeria Bertagnolo, Marco Marchisio, Sabina Pierpaoli, Maria Luisa Colamussi, Federica Brugnoli, Giuseppe Visani, Giorgio Zauli, Silvano Capitani

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we have investigated the expression of phospholipase C-β2 during the course of granulocytic differentiation of normal and malignant progenitors. As a model system, we used the NB4 cell line, a reliable in vitro model for the study of acute promyelocytic leukemia (APL), a variety of acute myeloid leukemia (AML) that responds to pharmacological doses of all transretinoic acid (ATRA) by differentiating in a neutrophil-like manner. We found that PLC-β2, virtually absent in untreated NB4 cells, was strongly upregulated after ATRA-induced granulocytic differentiation. Remarkably, using primary blasts purified from bone marrow of patients affected by APL successfully induced to remission by treatment with ATRA, we showed a striking correlation between the amount of PLC-β2 expression and the responsiveness of APL blasts to the differentiative activity of ATRA. An increase of PLC-β2 expression also characterized the cytokine-induced granulocytic differentiation of CD34+ normal hematopoietic progenitors. Taken together, these data show that PLC-β2 represents a sensitive and reliable marker of neutrophil maturation of normal and malignant myeloid progenitors. Moreover, PLC-β2 levels can predict the in vivo responsiveness to ATRA of APL patients.

Original languageEnglish
Pages (from-to)957-965
Number of pages9
JournalJournal of Leukocyte Biology
Volume71
Issue number6
Publication statusPublished - Jun 1 2002

Keywords

  • Cellular differentiation
  • Hematopoiesis
  • Lipid mediators
  • Neutrophils
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Selective up-regulation of phospholipase C-β2 during granulocytic differentiation of normal and leukemic hematopoietic progenitors'. Together they form a unique fingerprint.

Cite this