Selectivity release of the type ii decoy il-1 receptor

Simone Orlando, Nadia Polentarutti, Alberto Mantovani

Research output: Contribution to journalArticlepeer-review


The type II IL-1 receptor (IL-1RII) is a non-signalling molecule which acts as a decoy target for IL-1. Various signals (e.g. chemoattractants and phorbol ester) induce metalloprotease-mediated rapid shedding of the IL-1RII. The present study was designed to compare the susceptibility to shedding of the decoy IL-1RII vs the signalling IL-1RI. HEK 293 cells and COS cells were transfected with cDNAs encoding the human IL-1RI, IL-1RII or both. Slow spontaneous release and rapid phorbol ester-induced shedding were only observed for the decoy IL-1RII. Similarly, OVCAR-3 cells, which express substantial amounts of both IL-1RI and IL-1RII, only released the IL-1RII. These results indicate that the IL-RII, but not the IL-1RI, is susceptible to proteolytic shedding, a finding consistent with the decoy function of this molecule. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1001-1006
Number of pages6
Issue number7
Publication statusPublished - Jul 2000


  • IL-1 decoy receptor
  • Shedding
  • Soluble receptors

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy


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