Semaphorin 5A drives melanoma progression

role of Bcl-2, miR-204 and c-Myb

Simona D'Aguanno, Elisabetta Valentini, Maria Grazia Tupone, Marianna Desideri, Marta Di Martile, Manuela Spagnuolo, Simonetta Buglioni, Cristiana Ercolani, Italia Falcone, Marco De Dominici, Michele Milella, Maria Giulia Rizzo, Bruno Calabretta, Carlo Cota, Andrea Anichini, Daniela Trisciuoglio, Donatella Del Bufalo

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Melanoma, the most aggressive form of skin cancer, is characterized by high rates of metastasis, drug resistance and mortality. Here we investigated the role of Semaphorin 5A (Sema5A) on the properties associated with melanoma progression and the factors involved in Sema5A regulation. METHODS: Western blotting, qRT-PCR, Chromatin immunoprecipitation (ChIP) assay, immunohistochemistry of melanoma patient specimens and xenograft tissues, in vitro Transwell assay for cell migration and invasion evaluation, in vitro capillary-like structure formation analysis. RESULTS: A significant correlation of Sema5A mRNA expression and melanoma progression was observed by analyzing GEO profile dataset. Endogenous Sema5A protein was detected in 95% of human melanoma cell lines tested, in 70% of metastatic specimens from patients affected by melanoma, and 16% of in situ melanoma specimens showed a focal positivity. We demonstrated that Sema5A regulates in vitro cell migration and invasion and the formation of vasculogenic structures. We also found an increase of Sema5A at both mRNA and protein level after forced expression of Bcl-2. By use of transcriptional and proteasome inhibitors, we showed that Bcl-2 increases the stability of Sema5A mRNA and protein. Moreover, by ChIP we demonstrated that Sema5A expression is under the control of the transcription factor c-Myb and that c-Myb recruitment on Sema5A promoter is increased after Bcl-2 overexpression. Finally, a concomitant decrease in the expression of Sema5A, Bcl-2 and c-Myb proteins was observed in melanoma cells after miR-204 overexpression. CONCLUSION: Overall our data provide evidences supporting the role of Sema5A in melanoma progression and the involvement of Bcl-2, miR-204 and c-Myb in regulating its expression.

Original languageEnglish
Number of pages1
JournalJournal of experimental & clinical cancer research : CR
Volume37
Issue number1
DOIs
Publication statusPublished - Nov 19 2018

Fingerprint

Semaphorins
Melanoma
Chromatin Immunoprecipitation
Messenger RNA
Proto-Oncogene Proteins c-myb
Cell Migration Assays
Proteins
Proteasome Inhibitors
Skin Neoplasms
Drug Resistance
Heterografts
Cell Movement
Transcription Factors

Keywords

  • Bcl-2
  • c-Myb
  • Melanoma
  • miR-204
  • Semaphorin 5A

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Semaphorin 5A drives melanoma progression : role of Bcl-2, miR-204 and c-Myb. / D'Aguanno, Simona; Valentini, Elisabetta; Tupone, Maria Grazia; Desideri, Marianna; Di Martile, Marta; Spagnuolo, Manuela; Buglioni, Simonetta; Ercolani, Cristiana; Falcone, Italia; De Dominici, Marco; Milella, Michele; Rizzo, Maria Giulia; Calabretta, Bruno; Cota, Carlo; Anichini, Andrea; Trisciuoglio, Daniela; Del Bufalo, Donatella.

In: Journal of experimental & clinical cancer research : CR, Vol. 37, No. 1, 19.11.2018.

Research output: Contribution to journalArticle

D'Aguanno, S, Valentini, E, Tupone, MG, Desideri, M, Di Martile, M, Spagnuolo, M, Buglioni, S, Ercolani, C, Falcone, I, De Dominici, M, Milella, M, Rizzo, MG, Calabretta, B, Cota, C, Anichini, A, Trisciuoglio, D & Del Bufalo, D 2018, 'Semaphorin 5A drives melanoma progression: role of Bcl-2, miR-204 and c-Myb', Journal of experimental & clinical cancer research : CR, vol. 37, no. 1. https://doi.org/10.1186/s13046-018-0933-x
D'Aguanno, Simona ; Valentini, Elisabetta ; Tupone, Maria Grazia ; Desideri, Marianna ; Di Martile, Marta ; Spagnuolo, Manuela ; Buglioni, Simonetta ; Ercolani, Cristiana ; Falcone, Italia ; De Dominici, Marco ; Milella, Michele ; Rizzo, Maria Giulia ; Calabretta, Bruno ; Cota, Carlo ; Anichini, Andrea ; Trisciuoglio, Daniela ; Del Bufalo, Donatella. / Semaphorin 5A drives melanoma progression : role of Bcl-2, miR-204 and c-Myb. In: Journal of experimental & clinical cancer research : CR. 2018 ; Vol. 37, No. 1.
@article{85f8700fe5fb46f8a26b87a5d4109b05,
title = "Semaphorin 5A drives melanoma progression: role of Bcl-2, miR-204 and c-Myb",
abstract = "BACKGROUND: Melanoma, the most aggressive form of skin cancer, is characterized by high rates of metastasis, drug resistance and mortality. Here we investigated the role of Semaphorin 5A (Sema5A) on the properties associated with melanoma progression and the factors involved in Sema5A regulation. METHODS: Western blotting, qRT-PCR, Chromatin immunoprecipitation (ChIP) assay, immunohistochemistry of melanoma patient specimens and xenograft tissues, in vitro Transwell assay for cell migration and invasion evaluation, in vitro capillary-like structure formation analysis. RESULTS: A significant correlation of Sema5A mRNA expression and melanoma progression was observed by analyzing GEO profile dataset. Endogenous Sema5A protein was detected in 95{\%} of human melanoma cell lines tested, in 70{\%} of metastatic specimens from patients affected by melanoma, and 16{\%} of in situ melanoma specimens showed a focal positivity. We demonstrated that Sema5A regulates in vitro cell migration and invasion and the formation of vasculogenic structures. We also found an increase of Sema5A at both mRNA and protein level after forced expression of Bcl-2. By use of transcriptional and proteasome inhibitors, we showed that Bcl-2 increases the stability of Sema5A mRNA and protein. Moreover, by ChIP we demonstrated that Sema5A expression is under the control of the transcription factor c-Myb and that c-Myb recruitment on Sema5A promoter is increased after Bcl-2 overexpression. Finally, a concomitant decrease in the expression of Sema5A, Bcl-2 and c-Myb proteins was observed in melanoma cells after miR-204 overexpression. CONCLUSION: Overall our data provide evidences supporting the role of Sema5A in melanoma progression and the involvement of Bcl-2, miR-204 and c-Myb in regulating its expression.",
keywords = "Bcl-2, c-Myb, Melanoma, miR-204, Semaphorin 5A",
author = "Simona D'Aguanno and Elisabetta Valentini and Tupone, {Maria Grazia} and Marianna Desideri and {Di Martile}, Marta and Manuela Spagnuolo and Simonetta Buglioni and Cristiana Ercolani and Italia Falcone and {De Dominici}, Marco and Michele Milella and Rizzo, {Maria Giulia} and Bruno Calabretta and Carlo Cota and Andrea Anichini and Daniela Trisciuoglio and {Del Bufalo}, Donatella",
year = "2018",
month = "11",
day = "19",
doi = "10.1186/s13046-018-0933-x",
language = "English",
volume = "37",
journal = "Journal of Experimental and Clinical Cancer Research",
issn = "0392-9078",
publisher = "BioMed Central Ltd.",
number = "1",

}

TY - JOUR

T1 - Semaphorin 5A drives melanoma progression

T2 - role of Bcl-2, miR-204 and c-Myb

AU - D'Aguanno, Simona

AU - Valentini, Elisabetta

AU - Tupone, Maria Grazia

AU - Desideri, Marianna

AU - Di Martile, Marta

AU - Spagnuolo, Manuela

AU - Buglioni, Simonetta

AU - Ercolani, Cristiana

AU - Falcone, Italia

AU - De Dominici, Marco

AU - Milella, Michele

AU - Rizzo, Maria Giulia

AU - Calabretta, Bruno

AU - Cota, Carlo

AU - Anichini, Andrea

AU - Trisciuoglio, Daniela

AU - Del Bufalo, Donatella

PY - 2018/11/19

Y1 - 2018/11/19

N2 - BACKGROUND: Melanoma, the most aggressive form of skin cancer, is characterized by high rates of metastasis, drug resistance and mortality. Here we investigated the role of Semaphorin 5A (Sema5A) on the properties associated with melanoma progression and the factors involved in Sema5A regulation. METHODS: Western blotting, qRT-PCR, Chromatin immunoprecipitation (ChIP) assay, immunohistochemistry of melanoma patient specimens and xenograft tissues, in vitro Transwell assay for cell migration and invasion evaluation, in vitro capillary-like structure formation analysis. RESULTS: A significant correlation of Sema5A mRNA expression and melanoma progression was observed by analyzing GEO profile dataset. Endogenous Sema5A protein was detected in 95% of human melanoma cell lines tested, in 70% of metastatic specimens from patients affected by melanoma, and 16% of in situ melanoma specimens showed a focal positivity. We demonstrated that Sema5A regulates in vitro cell migration and invasion and the formation of vasculogenic structures. We also found an increase of Sema5A at both mRNA and protein level after forced expression of Bcl-2. By use of transcriptional and proteasome inhibitors, we showed that Bcl-2 increases the stability of Sema5A mRNA and protein. Moreover, by ChIP we demonstrated that Sema5A expression is under the control of the transcription factor c-Myb and that c-Myb recruitment on Sema5A promoter is increased after Bcl-2 overexpression. Finally, a concomitant decrease in the expression of Sema5A, Bcl-2 and c-Myb proteins was observed in melanoma cells after miR-204 overexpression. CONCLUSION: Overall our data provide evidences supporting the role of Sema5A in melanoma progression and the involvement of Bcl-2, miR-204 and c-Myb in regulating its expression.

AB - BACKGROUND: Melanoma, the most aggressive form of skin cancer, is characterized by high rates of metastasis, drug resistance and mortality. Here we investigated the role of Semaphorin 5A (Sema5A) on the properties associated with melanoma progression and the factors involved in Sema5A regulation. METHODS: Western blotting, qRT-PCR, Chromatin immunoprecipitation (ChIP) assay, immunohistochemistry of melanoma patient specimens and xenograft tissues, in vitro Transwell assay for cell migration and invasion evaluation, in vitro capillary-like structure formation analysis. RESULTS: A significant correlation of Sema5A mRNA expression and melanoma progression was observed by analyzing GEO profile dataset. Endogenous Sema5A protein was detected in 95% of human melanoma cell lines tested, in 70% of metastatic specimens from patients affected by melanoma, and 16% of in situ melanoma specimens showed a focal positivity. We demonstrated that Sema5A regulates in vitro cell migration and invasion and the formation of vasculogenic structures. We also found an increase of Sema5A at both mRNA and protein level after forced expression of Bcl-2. By use of transcriptional and proteasome inhibitors, we showed that Bcl-2 increases the stability of Sema5A mRNA and protein. Moreover, by ChIP we demonstrated that Sema5A expression is under the control of the transcription factor c-Myb and that c-Myb recruitment on Sema5A promoter is increased after Bcl-2 overexpression. Finally, a concomitant decrease in the expression of Sema5A, Bcl-2 and c-Myb proteins was observed in melanoma cells after miR-204 overexpression. CONCLUSION: Overall our data provide evidences supporting the role of Sema5A in melanoma progression and the involvement of Bcl-2, miR-204 and c-Myb in regulating its expression.

KW - Bcl-2

KW - c-Myb

KW - Melanoma

KW - miR-204

KW - Semaphorin 5A

UR - http://www.scopus.com/inward/record.url?scp=85056744328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056744328&partnerID=8YFLogxK

U2 - 10.1186/s13046-018-0933-x

DO - 10.1186/s13046-018-0933-x

M3 - Article

VL - 37

JO - Journal of Experimental and Clinical Cancer Research

JF - Journal of Experimental and Clinical Cancer Research

SN - 0392-9078

IS - 1

ER -