Semen preparation by standard swim-up versus swim-up with test yolk buffer incubation in intrauterine insemination: A randomized study

Guido Ragni, Luciana De Lauretis, Gerardo D'Ambrogio, Sandra Pellegrini, Paola Maggioni, Walter Vegetti, Laura Perotti, Alessandra Dalla Serra, Fabio Parazzini, Pier Giorgio Crosignani

Research output: Contribution to journalArticle

Abstract

In order to compare the standard swim-up semen preparation with and without test yolk buffer (TYB) incubation in intrauterine insemination (IUI), we conducted a prospective multicentre randomized trial. A total of 121 infertile couples with male factor (n = 52) or unexplained infertility (n = 69) was randomly assigned to two groups following ovulation induction. Semen was prepared by standard swim-up in group A (n = 64) and by swim-up followed by TYB incubation in group B (n = 57). A maximum of two IUI cycles was performed. A total of 104 cycles was performed in the swim-up group and 90 in the TYB group. Overall, 15 pregnancies were achieved in group A and 23 in group B, with an overall pregnancy rate of 24.8 and 50.0% per patient respectively (χ21, P <0.05). In the male factor group, pregnancy was achieved in six out of 24 couples (25%) following standard swim-up and in six out of 28 (21.4%) following swim-up and TYB incubation (χ21, not significant). In the unexplained infertility group, pregnancy was recorded in nine out of 40 couples (22.5%) following standard swim-up and in 17 out of 29 couples (58.6%) following swim-up and TYB incubation (χ21, P <0.05).

Original languageEnglish
Pages (from-to)1859-1863
Number of pages5
JournalHuman Reproduction
Volume13
Issue number7
Publication statusPublished - 1998

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Keywords

  • Intrauterine insemination
  • Randomized controlled trial
  • Semen preparation
  • Test yolk buffer
  • Unexplained infertility

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Ragni, G., De Lauretis, L., D'Ambrogio, G., Pellegrini, S., Maggioni, P., Vegetti, W., Perotti, L., Dalla Serra, A., Parazzini, F., & Crosignani, P. G. (1998). Semen preparation by standard swim-up versus swim-up with test yolk buffer incubation in intrauterine insemination: A randomized study. Human Reproduction, 13(7), 1859-1863.