Sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of vinorelbine plasma concentrations

I. Robieux; V. Vitali; P. Aita; A. Freschi; R. Lazzarini; R. Sorio

doi:10.1016/0378-4347(95)00332-0

Sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of vinorelbine plasma concentrations

I. Robieux, V. Vitali, P. Aita, A. Freschi, R. Lazzarini, R. Sorio

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

A high-performance liquid chromatographic (HPLC) method with fluorescence detection for the determination of vinorelbine in plasma is described. The technique was derived from that published by Debal for an assay of vinorelbine in cell culture medium. The modifications concern the preparation procedure for plasma samples (a two-step liquid-liquid extraction from plasma is described), optimization of the mobile phase composition, and use of a single C ₁₈ column. These changes resulted in an improved sensitivity and reproducibility of the assay and led to its feasibility for clinical pharmacokinetic studies. The range of the assay is 2 to 1000 ng/ml.

Original language	English
Pages (from-to)	183-187
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Applications
Volume	675
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(95)00332-0
Publication status	Published - Jan 12 1996

Keywords

Vinorelbine

ASJC Scopus subject areas

Chemistry(all)

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10.1016/0378-4347(95)00332-0

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Sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of vinorelbine plasma concentrations. / Robieux, I.; Vitali, V.; Aita, P.; Freschi, A.; Lazzarini, R.; Sorio, R.

In: Journal of Chromatography B: Biomedical Applications, Vol. 675, No. 1, 12.01.1996, p. 183-187.

Research output: Contribution to journal › Article › peer-review

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AU - Sorio, R.

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