Sensitivity of different resistant tumour cell lines to the two novel compounds (2Z,4E)-2-methylsulfanyl-5-(1-naphthyl)-4-nitro-2,4-pentadienoate and (1E,3E)-1,4-bis(2-naphthyl)-2,3-dinitro-1,3-butadiene

Maurizio Viale, Giovanni Petrillo, Massimo Maccagno, Patrizio Castagnola, Cinzia Aiello, Cinzia Cordazzo, Maria A. Mariggiò, Sushilkumar A. Jadhav, Lara Bianchi, Giuseppe Leto, Egon Rizzato, Alessandro Poggi, Domenico Spinelli

Research output: Contribution to journalArticle

Abstract

The inhibition of cell proliferation by methyl (2Z,4E)-2-methylsulfanyl-5-(1-naphthyl)-4-nitro-2,4-pentadienoate (1-Naph-NMCB) and (1E,3E)-1,4-bis(2-naphthyl)-2,3-dinitro-1,3-butadiene (2-Naph-DNB) has been studied in vitro against four cell lines selected for their resistance to doxorubicin, cisplatin, taxol and 5-fluorouracil. In previous experiments both compounds showed good in vitro antiproliferative, cytotoxic and pro-apoptotic activities against cell lines of different histologic origin. The results of the experiments presented here suggest that 1-Naph-NMCB is able to overcome all of the different mechanisms of resistance showed by the resistant cell lines used for our experiments. On the contrary, when we used the taxol-resistant A549-T12 cell line, characterized by a mechanism of resistance due to a mutation of the target site of taxol on microtubules, it displayed a partial but significant cross-resistance to 2-Naph-DNB. Although the actual mechanism of this cross-resistance has not yet been definitively elucidated, our results from immunostaining of microtubules suggest that it may be linked to the presence of a shared target site for taxol and 2-Naph-DNB on microtubules.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalEuropean Journal of Pharmacology
Volume588
Issue number1
DOIs
Publication statusPublished - Jun 24 2008

Keywords

  • 1-Naph-NMCB
  • 2-Naph-DNB
  • Drug-resistance
  • Mechanism of resistance

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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