Sensitivity of two enzyme-linked immunosorbent assay tests in relation to western blot in detecting human T-cell lymphotropic virus types I and II infection among HIV-1 infected patients from Sao Paulo, Brazil

Adele Caterino-de-Araujo, Elizabeth De los Santos-Fortuna, Mônica Cristina Zandoná Meleiro, Jamal Suleiman, Maria Luisa Calabrò, Anna Favero, Anita De Rossi, Luigi Chieco-Bianchi

Research output: Contribution to journalArticle

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Abstract

We investigated the presence of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II) infections, first searching for specific antibodies in 553 serum samples obtained from HIV-1-infected patients from Sao Paulo, Brazil. Sera were screened using two enzyme-linked immunosorbent assays (ELISAs): the ELISA-EM (ELISA HTLV-I/II, EMBRABIO, BR), which contains HTLV-I and HTLV-II lysates, and the ELISA-DB [ELISA HTLV-I/II, Diagnostic Biotechnology (DB), Singapore], which contains HTLV-I lysate, and HTL V-I and HTL V-II recombinant env proteins (MTA-1 and K55, respectively). Serum samples showing two positive and/or borderline results were confirmed by Western blot (WB 2.3, DB), which discriminates HTLV-I from HTLV-II. WB analyses disclosed 22 cases (4.0%) of HTLV-I and 34 (6.1%) of HTL V-II seroreactivity; 24 sera had indeterminate antibody profile (4.3%) and 2 specimens showed reactivity to both MTA-1 and K55 env proteins. Using stringent WB criteria and analyzing the population according to risk factors, the prevalence rates of HTLV-I and HTLV-II infections were 11.2% and 16.8% in IV drug users, 3.4% and 5.5% in heterosexual individuals, and 1.4% and 2.2% in homosexual/bisexual men, respectively. A comparison of ELISA and WB results disclosed that both ELISAs were highly sensitive in detecting HTLV-I antibodies, whereas the ELISA-DB showed 82% sensitivity and the ELISA-EM 100% sensitivity in detecting HTL V-II antibodies. PCR analyses conducted on 37 representative cells samples confirmed the presence of HTL V proviral DNA in the majority of concordant serological cases, except in one, which was HTL V- I infected and seroreacted with K55 protein of HTLV-II. Indeed, after PCR, one case of HTL V-I infection and HTL V-II coinfection, and 30% of WB- seroindeterminate or inconclusive cases infected with HTL V-II could be detected. Our data stress high prevalences of both HTL V-I and HTL V-II infections in HIV-1 coinfected i.v. drug users from Sao Paulo, and suggests that ELISA kits containing only K55 protein as the HTL V-II-specific antigen, may not have the appropriate sensitivity for the detection of HTLV-II infection in this geographic region, pointing out the need of improved screening tests to be used in Brazil.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalDiagnostic Microbiology and Infectious Disease
Volume30
Issue number3
DOIs
Publication statusPublished - 1998

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Human T-lymphotropic virus 2
Human T-lymphotropic virus 1
Brazil
HIV-1
Western Blotting
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Infection
HTLV-II Infections
Biotechnology
Pemetrexed
env Gene Products
Drug Users
Serum
Antibodies
HTLV-I Antibodies
Polymerase Chain Reaction
Singapore
Heterosexuality
Coinfection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Sensitivity of two enzyme-linked immunosorbent assay tests in relation to western blot in detecting human T-cell lymphotropic virus types I and II infection among HIV-1 infected patients from Sao Paulo, Brazil. / Caterino-de-Araujo, Adele; De los Santos-Fortuna, Elizabeth; Meleiro, Mônica Cristina Zandoná; Suleiman, Jamal; Calabrò, Maria Luisa; Favero, Anna; De Rossi, Anita; Chieco-Bianchi, Luigi.

In: Diagnostic Microbiology and Infectious Disease, Vol. 30, No. 3, 1998, p. 173-182.

Research output: Contribution to journalArticle

Caterino-de-Araujo, Adele ; De los Santos-Fortuna, Elizabeth ; Meleiro, Mônica Cristina Zandoná ; Suleiman, Jamal ; Calabrò, Maria Luisa ; Favero, Anna ; De Rossi, Anita ; Chieco-Bianchi, Luigi. / Sensitivity of two enzyme-linked immunosorbent assay tests in relation to western blot in detecting human T-cell lymphotropic virus types I and II infection among HIV-1 infected patients from Sao Paulo, Brazil. In: Diagnostic Microbiology and Infectious Disease. 1998 ; Vol. 30, No. 3. pp. 173-182.
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abstract = "We investigated the presence of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II) infections, first searching for specific antibodies in 553 serum samples obtained from HIV-1-infected patients from Sao Paulo, Brazil. Sera were screened using two enzyme-linked immunosorbent assays (ELISAs): the ELISA-EM (ELISA HTLV-I/II, EMBRABIO, BR), which contains HTLV-I and HTLV-II lysates, and the ELISA-DB [ELISA HTLV-I/II, Diagnostic Biotechnology (DB), Singapore], which contains HTLV-I lysate, and HTL V-I and HTL V-II recombinant env proteins (MTA-1 and K55, respectively). Serum samples showing two positive and/or borderline results were confirmed by Western blot (WB 2.3, DB), which discriminates HTLV-I from HTLV-II. WB analyses disclosed 22 cases (4.0{\%}) of HTLV-I and 34 (6.1{\%}) of HTL V-II seroreactivity; 24 sera had indeterminate antibody profile (4.3{\%}) and 2 specimens showed reactivity to both MTA-1 and K55 env proteins. Using stringent WB criteria and analyzing the population according to risk factors, the prevalence rates of HTLV-I and HTLV-II infections were 11.2{\%} and 16.8{\%} in IV drug users, 3.4{\%} and 5.5{\%} in heterosexual individuals, and 1.4{\%} and 2.2{\%} in homosexual/bisexual men, respectively. A comparison of ELISA and WB results disclosed that both ELISAs were highly sensitive in detecting HTLV-I antibodies, whereas the ELISA-DB showed 82{\%} sensitivity and the ELISA-EM 100{\%} sensitivity in detecting HTL V-II antibodies. PCR analyses conducted on 37 representative cells samples confirmed the presence of HTL V proviral DNA in the majority of concordant serological cases, except in one, which was HTL V- I infected and seroreacted with K55 protein of HTLV-II. Indeed, after PCR, one case of HTL V-I infection and HTL V-II coinfection, and 30{\%} of WB- seroindeterminate or inconclusive cases infected with HTL V-II could be detected. Our data stress high prevalences of both HTL V-I and HTL V-II infections in HIV-1 coinfected i.v. drug users from Sao Paulo, and suggests that ELISA kits containing only K55 protein as the HTL V-II-specific antigen, may not have the appropriate sensitivity for the detection of HTLV-II infection in this geographic region, pointing out the need of improved screening tests to be used in Brazil.",
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AU - De los Santos-Fortuna, Elizabeth

AU - Meleiro, Mônica Cristina Zandoná

AU - Suleiman, Jamal

AU - Calabrò, Maria Luisa

AU - Favero, Anna

AU - De Rossi, Anita

AU - Chieco-Bianchi, Luigi

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