Separazione e caratterizzazione delle popolazioni linfocitarie TG e TM nell'uomo.

Translated title of the contribution: Separation and characterization of human T G and T M lymphocyte populations

E. Mariani, A. R. Mariani, M. Mazzetti, M. Mazzoli, M. Baraldini, A. Facchini, G. F. Stefanini

Research output: Contribution to journalArticlepeer-review

Abstract

During the last few years a number of experimental evidences have shown the presence of Fc receptors for IgG or IgM on the membrane of human T cells. These two different receptors are detectable and mutually exclusive on distinct cell populations named respectively TG, TM and T "null" (which lack detectable receptors). Studies on the functional activities of these cells have shown that TM and TG lymphocytes play an antitetical role in regulating B cell response, TM exerting an "helper" activity on the differentiation of B lymphocytes while TG having a "suppressor" one. The aim of this study has been to determine the values of these two subpopulations in a group of twenty control subjects. Our results have shown that TG constitute 10%, whereas TM represent 40% of the total T cells. After EA-G rosetting, the purification of this subpopulation on a density gradient has shown an enrichment of more than 90% in TG cells, while TM contaminate this fraction for less than 4%. The purity of the fraction containing TM has been evaluated using the localization of alpha-naphthyl acetate esterase activity, which has shown that more than 88% of the cells in this fraction are positive for this enzyme.

Translated title of the contributionSeparation and characterization of human T G and T M lymphocyte populations
Original languageItalian
Pages (from-to)1590-1596
Number of pages7
JournalBollettino della Societa Italiana di Biologia Sperimentale
Volume56
Issue number15
Publication statusPublished - Aug 15 1980

ASJC Scopus subject areas

  • Medicine(all)

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