Separation of dansylamino acid enantiomers by thin-layer chromatography

Massimo Sinibaldi, Antonella Messina, Anna Maria Girelli

Research output: Contribution to journalArticlepeer-review

Abstract

A simple thin-layer chromatographic method for the separation of D- and L-dansylamino acids using a new resolving agent is described. The enantiomeric resolution of racemates was accomplished on reversed-phase plates pre-treated with a poly-L-phenylalaninamide - copper(II) complex using water- acetonitrile mixtures as eluent under isocratic conditions. The polymeric ligand was easily prepared by reaction of the optically active amide with ethylene glycol diglycidyl ether at room temperature. The method is rapid and sensitive, and was successfully employed to resolve various dansylamino acids.

Original languageEnglish
Pages (from-to)1245-1247
Number of pages3
JournalAnalyst
Volume113
DOIs
Publication statusPublished - 1988

Keywords

  • Dansylamino acids
  • Ligand-exchange chromatography
  • Polymeric resolving agent
  • Racemate resolution
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry

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