Separation of rat pituitary growth hormone and prolactin by SDS polyacrylamide gel electrophoresis

A. Zanini, G. Giannattasio, J. Meldolesi

Research output: Contribution to journalArticlepeer-review

Abstract

Isolation of growth hormone (GH) and prolactin (PRL) from rat pituitary homogenates and cell fractions was carried out by polyacrylamide gel electrophoresis using a high resolution Na dodecylsulfate (SDS) system. With this technique GH and PRL are resolved as discrete and well separated bands. The method appears particularly adequate for quantitative determinations of the hormones and of the hormone bound radioactivity not only in pituitary homogenates but also in isolated cell fractions, since the treatment with SDS results in the complete solubilization of all samples and therefore allows the electrophoretic migrations of all hormone molecules. Using the SDS gel system, it was also possible to estimate the degree of contamination by nonhormone proteins in GH and PRL bands separated by the usual method of Ornstein and Davis.

Original languageEnglish
Pages (from-to)594-598
Number of pages5
JournalEndocrinology
Volume94
Issue number2
Publication statusPublished - 1974

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Separation of rat pituitary growth hormone and prolactin by SDS polyacrylamide gel electrophoresis'. Together they form a unique fingerprint.

Cite this