Separation of tocopherols by nano-liquid chromatography

Salvatore Fanali, Emanuela Camera, Bezhan Chankvetadze, Giovanni D'Orazio, Maria Giovanna Quaglia

Research output: Contribution to journalArticle


Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100μm I.D. and 375μm O.D. packed in our laboratory with RP18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6-9min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest Rs and N/m values were observed using 3-μm RP18 particles. Experiments performed with capillaries packed with 3-μm RP18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8μg/ml, while for α-TOH were 6 and 10μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number2
Publication statusPublished - Apr 16 2004


  • α-tocopherol acetate
  • α-TOH-Ac
  • Drugs
  • Nano-LC
  • Nano-liquid chromatography
  • Serum
  • Tocopherols
  • TOHs
  • Vitamin E

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Separation of tocopherols by nano-liquid chromatography'. Together they form a unique fingerprint.

  • Cite this