Sepharose-bound, highly sulfated glycosaminoglycans can capture HIV-1 from culture medium

Barbara Curatella, Barbara Bartolini, Antonino Di Caro, Rosaria A. Cavallaro, Lino Liverani, Giuseppe Mascellani, Arrigo Benedetto, Concetta Castilletti, Maria R. Capobianchi, Luciano Cellai

Research output: Contribution to journalArticlepeer-review

Abstract

In the search for new strategies against HIV-1 and on the basis of a number of previous studies reporting on the capacity of certain polyanionic compounds to influence the replication of HIV-1, we prepared a few chemically oversulfated dermatan and chondroitin sulfates. Four of these compounds and two samples of heparin were bound to activated Sepharose through either their carboxylic groups, or their aldehydic groups, or their deacetylated primary amino groups. Some of these so-derivatised resins, packed into columns, proved able to remove HIV-1 IIIB, a laboratory adapted strain, and one clinical primary isolate from an AIDS patient, from infected cell culture medium. The resins bind the virus very tightly and could be useful for capturing the virus from infected fluids.

Original languageEnglish
Pages (from-to)759-764
Number of pages6
JournalCarbohydrate Research
Volume340
Issue number4
DOIs
Publication statusPublished - Mar 21 2005

Keywords

  • Chondroitin sulfate
  • Dermatan sulfate
  • Heparin
  • HIV-1
  • Oversulfated glycosaminoglycans

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry

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