Sequence analysis of serum albumins reveals the molecular evolution of ligand recognition properties

Gabriella Fanali, Paolo Ascenzi, Giorgio Bernardi, Mauro Fasano

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Serum albumin (SA) is a circulating protein providing a depot and carrier for many endogenous and exogenous compounds. At least seven major binding sites have been identified by structural and functional investigations mainly in human SA. SA is conserved in vertebrates, with at least 49 entries in protein sequence databases. The multiple sequence analysis of this set of entries leads to the definition of a cladistic tree for the molecular evolution of SA orthologs in vertebrates, thus showing the clustering of the considered species, with lamprey SAs (Lethenteron japonicum and Petromyzon marinus) in a separate outgroup. Sequence analysis aimed at searching conserved domains revealed that most SA sequences are made up by three repeated domains (about 600 residues), as extensively characterized for human SA. On the contrary, lamprey SAs are giant proteins (about 1400 residues) comprising seven repeated domains. The phylogenetic analysis of the SA family reveals a stringent correlation with the taxonomic classification of the species available in sequence databases. A focused inspection of the sequences of ligand binding sites in SA revealed that in all sites most residues involved in ligand binding are conserved, although the versatility towards different ligands could be peculiar of higher organisms. Moreover, the analysis of molecular links between the different sites suggests that allosteric modulation mechanisms could be restricted to higher vertebrates.

Original languageEnglish
Pages (from-to)1195-1205
Number of pages11
JournalJournal of Biomolecular Structure and Dynamics
Volume29
Issue number6
DOIs
Publication statusPublished - 2012

Fingerprint

Molecular Evolution
Serum Albumin
Sequence Analysis
Ligands
Lampreys
Vertebrates
Petromyzon
Binding Sites
Allosteric Site
Protein Databases
Cluster Analysis
Proteins
Databases

Keywords

  • Albumin
  • Allostery
  • Ligand binding
  • Molecular evolution
  • Structural evolution

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Sequence analysis of serum albumins reveals the molecular evolution of ligand recognition properties. / Fanali, Gabriella; Ascenzi, Paolo; Bernardi, Giorgio; Fasano, Mauro.

In: Journal of Biomolecular Structure and Dynamics, Vol. 29, No. 6, 2012, p. 1195-1205.

Research output: Contribution to journalArticle

Fanali, Gabriella ; Ascenzi, Paolo ; Bernardi, Giorgio ; Fasano, Mauro. / Sequence analysis of serum albumins reveals the molecular evolution of ligand recognition properties. In: Journal of Biomolecular Structure and Dynamics. 2012 ; Vol. 29, No. 6. pp. 1195-1205.
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