Sequence and characterisation of the RET proto-oncogene 5' flanking region: Analysis of retinoic acid responsiveness at the transcriptional level

Giovanna Patrone; Aldamaria Puliti; Renata Bocciardi; Roberto Ravazzolo; Giovanni Romeo

doi:10.1016/S0014-5793(97)01435-X

Sequence and characterisation of the RET proto-oncogene 5' flanking region: Analysis of retinoic acid responsiveness at the transcriptional level

Giovanna Patrone, Aldamaria Puliti, Renata Bocciardi, Roberto Ravazzolo, Giovanni Romeo

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up- regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.

Original language	English
Pages (from-to)	76-82
Number of pages	7
Journal	FEBS Letters
Volume	419
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)01435-X
Publication status	Published - Dec 8 1997

Keywords

Hirschsprung
Medullary thyroid carcinoma
Multiple endocrine neoplasia type 2
Neuroblastoma
RET proto-oncogene
Retinoic acid response element

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(97)01435-X

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abstract = "The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up- regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.",

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T2 - Analysis of retinoic acid responsiveness at the transcriptional level

AU - Patrone, Giovanna

AU - Puliti, Aldamaria

AU - Bocciardi, Renata

AU - Ravazzolo, Roberto

AU - Romeo, Giovanni

PY - 1997/12/8

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