TY - JOUR
T1 - Sequence and characterisation of the RET proto-oncogene 5' flanking region
T2 - Analysis of retinoic acid responsiveness at the transcriptional level
AU - Patrone, Giovanna
AU - Puliti, Aldamaria
AU - Bocciardi, Renata
AU - Ravazzolo, Roberto
AU - Romeo, Giovanni
PY - 1997/12/8
Y1 - 1997/12/8
N2 - The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up- regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.
AB - The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up- regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.
KW - Hirschsprung
KW - Medullary thyroid carcinoma
KW - Multiple endocrine neoplasia type 2
KW - Neuroblastoma
KW - RET proto-oncogene
KW - Retinoic acid response element
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U2 - 10.1016/S0014-5793(97)01435-X
DO - 10.1016/S0014-5793(97)01435-X
M3 - Article
C2 - 9426223
AN - SCOPUS:0031449935
VL - 419
SP - 76
EP - 82
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -