Sequence and characterisation of the RET proto-oncogene 5' flanking region: Analysis of retinoic acid responsiveness at the transcriptional level

Giovanna Patrone, Aldamaria Puliti, Renata Bocciardi, Roberto Ravazzolo, Giovanni Romeo

Research output: Contribution to journalArticlepeer-review

Abstract

The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up- regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalFEBS Letters
Volume419
Issue number1
DOIs
Publication statusPublished - Dec 8 1997

Keywords

  • Hirschsprung
  • Medullary thyroid carcinoma
  • Multiple endocrine neoplasia type 2
  • Neuroblastoma
  • RET proto-oncogene
  • Retinoic acid response element

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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