TY - JOUR
T1 - Sequence and TLR9 independent increase of TRACP expression by antisense DNA and siRNA molecules
AU - Muhonen, Pirkko
AU - Avnet, Sofia
AU - Parthasarathy, Ranga N.
AU - Janckila, Anthony J.
AU - Halleen, Jussi M.
AU - Laitala-Leinonen, Tiina
AU - Väänänen, H. Kalervo
PY - 2007/8/10
Y1 - 2007/8/10
N2 - Reactive oxygen species generating activity of tartrate-resistant acid phosphatase (TRACP) has been suggested to have several functions in TRACP expressing bone resorbing osteoclasts, macrophages, and dendritic cells. This work aimed to study the TRACP knock down phenotype in osteoclasts by using antisense DNA and RNA interference methods. Unexpectedly, both TRACP specific DNA oligonucleotides and siRNA molecules extensively increased the TRACP expression in human osteoclasts and monocytes. Toll-like receptor 9 (TLR9) is an immunity sensor for CpG motifs in DNA. We cultured bone marrow-derived osteoclast precursor cells from wild-type and TLR9-/- mice with CpG and non-CpG DNA oligonucleotides, and observed that the increased TRACP expression was sequence and TLR9 independent. In contrast, cells with increased TRACP activity showed decreased activity of tartrate-sensitive acid phosphatases. Conclusion: DNA oligonucleotides and RNA molecules extensively increase TRACP expression in monocyte-macrophage lineage. These results suggest a potential role of TRACP in pathogen recognition and in innate immunity.
AB - Reactive oxygen species generating activity of tartrate-resistant acid phosphatase (TRACP) has been suggested to have several functions in TRACP expressing bone resorbing osteoclasts, macrophages, and dendritic cells. This work aimed to study the TRACP knock down phenotype in osteoclasts by using antisense DNA and RNA interference methods. Unexpectedly, both TRACP specific DNA oligonucleotides and siRNA molecules extensively increased the TRACP expression in human osteoclasts and monocytes. Toll-like receptor 9 (TLR9) is an immunity sensor for CpG motifs in DNA. We cultured bone marrow-derived osteoclast precursor cells from wild-type and TLR9-/- mice with CpG and non-CpG DNA oligonucleotides, and observed that the increased TRACP expression was sequence and TLR9 independent. In contrast, cells with increased TRACP activity showed decreased activity of tartrate-sensitive acid phosphatases. Conclusion: DNA oligonucleotides and RNA molecules extensively increase TRACP expression in monocyte-macrophage lineage. These results suggest a potential role of TRACP in pathogen recognition and in innate immunity.
KW - CpG motif
KW - DNA antisense
KW - Innate immunity
KW - siRNA
KW - TLR9
KW - TRACP
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UR - http://www.scopus.com/inward/citedby.url?scp=34250699499&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.05.205
DO - 10.1016/j.bbrc.2007.05.205
M3 - Article
C2 - 17570345
AN - SCOPUS:34250699499
VL - 359
SP - 889
EP - 895
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -