Sequence and TLR9 independent increase of TRACP expression by antisense DNA and siRNA molecules

Pirkko Muhonen, Sofia Avnet, Ranga N. Parthasarathy, Anthony J. Janckila, Jussi M. Halleen, Tiina Laitala-Leinonen, H. Kalervo Väänänen

Research output: Contribution to journalArticlepeer-review

Abstract

Reactive oxygen species generating activity of tartrate-resistant acid phosphatase (TRACP) has been suggested to have several functions in TRACP expressing bone resorbing osteoclasts, macrophages, and dendritic cells. This work aimed to study the TRACP knock down phenotype in osteoclasts by using antisense DNA and RNA interference methods. Unexpectedly, both TRACP specific DNA oligonucleotides and siRNA molecules extensively increased the TRACP expression in human osteoclasts and monocytes. Toll-like receptor 9 (TLR9) is an immunity sensor for CpG motifs in DNA. We cultured bone marrow-derived osteoclast precursor cells from wild-type and TLR9-/- mice with CpG and non-CpG DNA oligonucleotides, and observed that the increased TRACP expression was sequence and TLR9 independent. In contrast, cells with increased TRACP activity showed decreased activity of tartrate-sensitive acid phosphatases. Conclusion: DNA oligonucleotides and RNA molecules extensively increase TRACP expression in monocyte-macrophage lineage. These results suggest a potential role of TRACP in pathogen recognition and in innate immunity.

Original languageEnglish
Pages (from-to)889-895
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number4
DOIs
Publication statusPublished - Aug 10 2007

Keywords

  • CpG motif
  • DNA antisense
  • Innate immunity
  • siRNA
  • TLR9
  • TRACP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Sequence and TLR9 independent increase of TRACP expression by antisense DNA and siRNA molecules'. Together they form a unique fingerprint.

Cite this