Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans

Stefano Buttò, Valeria Fiorelli, Antonella Tripiciano, Maria J. Ruiz-Alvarez, Arianna Scoglio, Fabrizio Ensoli, Massimo Ciccozzi, Barbara Collacchi, Michela Sabbatucci, Aurelio Cafaro, Carlos A. Guzmán, Alessandra Borsetti, Antonella Caputo, Eftyhia Vardas, Mark Colvin, Matthew Lukwiya, Giovanni Rezza, Barbara Ensoli

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Abstract

We determined immune cross-recognition and the degree of Tat conservation in patients infected by local human immunodeficiency virus (HIV) type 1 strains. The data indicated a similar prevalence of total and epitope-specific anti-Tat IgG in 578 serum samples from HIV-infected Italian (n = 302), Ugandan (n = 139), and South African (n = 137) subjects, using the same B clade Tat protein that is being used in vaccine trials. In particular, anti-Tat antibodies were detected in 13.2%, 10.8%, and 13.9% of HIV-1-infected individuals from Italy, Uganda, and South Africa, respectively. Sequence analysis results indicated a high similarity of Tat from the different circulating viruses with BH-10 Tat, particularly in the 1-58 amino acid region, which contains most of the immunogenic epitopes. These data indicate an effective cross-recognition of a B-clade laboratory strain-derived Tat protein vaccine by individuals infected with different local viruses, owing to the high similarity of Tat epitopes.

Original languageEnglish
Pages (from-to)1171-1180
Number of pages10
JournalJournal of Infectious Diseases
Volume188
Issue number8
DOIs
Publication statusPublished - Oct 15 2003

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tat Gene Products
Cercopithecine Herpesvirus 1
HIV-1
Epitopes
Antibodies
Vaccines
Viruses
Uganda
South Africa
Italy
Sequence Analysis
Anti-Idiotypic Antibodies
HIV
Amino Acids
Serum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans. / Buttò, Stefano; Fiorelli, Valeria; Tripiciano, Antonella; Ruiz-Alvarez, Maria J.; Scoglio, Arianna; Ensoli, Fabrizio; Ciccozzi, Massimo; Collacchi, Barbara; Sabbatucci, Michela; Cafaro, Aurelio; Guzmán, Carlos A.; Borsetti, Alessandra; Caputo, Antonella; Vardas, Eftyhia; Colvin, Mark; Lukwiya, Matthew; Rezza, Giovanni; Ensoli, Barbara.

In: Journal of Infectious Diseases, Vol. 188, No. 8, 15.10.2003, p. 1171-1180.

Research output: Contribution to journalArticle

Buttò, S, Fiorelli, V, Tripiciano, A, Ruiz-Alvarez, MJ, Scoglio, A, Ensoli, F, Ciccozzi, M, Collacchi, B, Sabbatucci, M, Cafaro, A, Guzmán, CA, Borsetti, A, Caputo, A, Vardas, E, Colvin, M, Lukwiya, M, Rezza, G & Ensoli, B 2003, 'Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans', Journal of Infectious Diseases, vol. 188, no. 8, pp. 1171-1180. https://doi.org/10.1086/378412
Buttò, Stefano ; Fiorelli, Valeria ; Tripiciano, Antonella ; Ruiz-Alvarez, Maria J. ; Scoglio, Arianna ; Ensoli, Fabrizio ; Ciccozzi, Massimo ; Collacchi, Barbara ; Sabbatucci, Michela ; Cafaro, Aurelio ; Guzmán, Carlos A. ; Borsetti, Alessandra ; Caputo, Antonella ; Vardas, Eftyhia ; Colvin, Mark ; Lukwiya, Matthew ; Rezza, Giovanni ; Ensoli, Barbara. / Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans. In: Journal of Infectious Diseases. 2003 ; Vol. 188, No. 8. pp. 1171-1180.
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