Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans

Stefano Buttò, Valeria Fiorelli, Antonella Tripiciano, Maria J. Ruiz-Alvarez, Arianna Scoglio, Fabrizio Ensoli, Massimo Ciccozzi, Barbara Collacchi, Michela Sabbatucci, Aurelio Cafaro, Carlos A. Guzmán, Alessandra Borsetti, Antonella Caputo, Eftyhia Vardas, Mark Colvin, Matthew Lukwiya, Giovanni Rezza, Barbara Ensoli

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Abstract

We determined immune cross-recognition and the degree of Tat conservation in patients infected by local human immunodeficiency virus (HIV) type 1 strains. The data indicated a similar prevalence of total and epitope-specific anti-Tat IgG in 578 serum samples from HIV-infected Italian (n = 302), Ugandan (n = 139), and South African (n = 137) subjects, using the same B clade Tat protein that is being used in vaccine trials. In particular, anti-Tat antibodies were detected in 13.2%, 10.8%, and 13.9% of HIV-1-infected individuals from Italy, Uganda, and South Africa, respectively. Sequence analysis results indicated a high similarity of Tat from the different circulating viruses with BH-10 Tat, particularly in the 1-58 amino acid region, which contains most of the immunogenic epitopes. These data indicate an effective cross-recognition of a B-clade laboratory strain-derived Tat protein vaccine by individuals infected with different local viruses, owing to the high similarity of Tat epitopes.

Original languageEnglish
Pages (from-to)1171-1180
Number of pages10
JournalJournal of Infectious Diseases
Volume188
Issue number8
DOIs
Publication statusPublished - Oct 15 2003

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Buttò, S., Fiorelli, V., Tripiciano, A., Ruiz-Alvarez, M. J., Scoglio, A., Ensoli, F., Ciccozzi, M., Collacchi, B., Sabbatucci, M., Cafaro, A., Guzmán, C. A., Borsetti, A., Caputo, A., Vardas, E., Colvin, M., Lukwiya, M., Rezza, G., & Ensoli, B. (2003). Sequence Conservation and Antibody Cross-Recognition of Clade B Human Immunodeficiency Virus (HIV) Type 1 Tat Protein in HIV-1-Infected Italians, Ugandans, and South Africans. Journal of Infectious Diseases, 188(8), 1171-1180. https://doi.org/10.1086/378412