Sequence-directed curvature of repetitive AluI DNA in constitutive heterochromatin of Artemia franciscana

Robert Benfante, Nicoletta Landsberger, Grazia Tubiello, Gianfranco Badaracco

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An Alu I family of repeated DNA sequence 113 bp in length was found to be the major component of the heterochromatin in Artemia franciscana. On the basis of the analysis of cloned oligomeric (monomer to examer) heterochromatic fragments we predicted that the sequence could produce a stable curvature in chromosomal DNA. This prediction was confirmed by polyacrylamide gel electrophoresls analysis and by electron microscope observations. The anomalous mobility of these fragments is reversed when the DNA samples are electrophoresed in the presence of distamycin A. Moreover treatment of living Artemia with this drug produces visible decondensatlon of heterochromatic masses in the interphase nuclei.

Original languageEnglish
Pages (from-to)8273-8282
Number of pages10
JournalNucleic Acids Research
Volume17
Issue number20
DOIs
Publication statusPublished - Oct 25 1989

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Dolphins
Artemia
Heterochromatin
Fragment
DNA
Curvature
Alu Elements
DNA sequences
Interphase
Polyacrylates
DNA Sequence
Microscope
Nucleus
Anomalous
Drugs
Electron microscopes
Gels
Monomers
Electrons
Electron

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Sequence-directed curvature of repetitive AluI DNA in constitutive heterochromatin of Artemia franciscana. / Benfante, Robert; Landsberger, Nicoletta; Tubiello, Grazia; Badaracco, Gianfranco.

In: Nucleic Acids Research, Vol. 17, No. 20, 25.10.1989, p. 8273-8282.

Research output: Contribution to journalArticle

Benfante, Robert ; Landsberger, Nicoletta ; Tubiello, Grazia ; Badaracco, Gianfranco. / Sequence-directed curvature of repetitive AluI DNA in constitutive heterochromatin of Artemia franciscana. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 20. pp. 8273-8282.
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