Abstract
Sequence-directed recognition peptides (SDRPs) were constructed on the basis of their hydropathic complementarity for big-endothelin (bigET). These peptides can inhibit in vitro the proteolytic cleavage that generates endothelin (ET) from its bigET precursor. Comparison of dissociation constants of the complexes SDRP:bigET with kinetic constants obtained for the cleavage of bigET by α-chymotrypsin (taken as a model proteinase) provides evidence of the potential of SDRPs. This is a novel application of SDRPs used as inhibitors of a proteolytic reaction.
| Original language | English |
|---|---|
| Pages (from-to) | 337-340 |
| Number of pages | 4 |
| Journal | Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular |
| Volume | 1202 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 6 1993 |
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Keywords
- big-Endothelin
- Complementary peptide
- Endothelin biosynthesis
- Substrate depletion
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Structural Biology
- Medicine(all)
Cite this
Sequence-directed recognition peptides : inhibition of endothelin generation via a substrate-depletion mechanism. / Zamai, Moreno; Caiolfa, Valeria R.
In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1202, No. 2, 06.10.1993, p. 337-340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence-directed recognition peptides
T2 - inhibition of endothelin generation via a substrate-depletion mechanism
AU - Zamai, Moreno
AU - Caiolfa, Valeria R.
PY - 1993/10/6
Y1 - 1993/10/6
N2 - Sequence-directed recognition peptides (SDRPs) were constructed on the basis of their hydropathic complementarity for big-endothelin (bigET). These peptides can inhibit in vitro the proteolytic cleavage that generates endothelin (ET) from its bigET precursor. Comparison of dissociation constants of the complexes SDRP:bigET with kinetic constants obtained for the cleavage of bigET by α-chymotrypsin (taken as a model proteinase) provides evidence of the potential of SDRPs. This is a novel application of SDRPs used as inhibitors of a proteolytic reaction.
AB - Sequence-directed recognition peptides (SDRPs) were constructed on the basis of their hydropathic complementarity for big-endothelin (bigET). These peptides can inhibit in vitro the proteolytic cleavage that generates endothelin (ET) from its bigET precursor. Comparison of dissociation constants of the complexes SDRP:bigET with kinetic constants obtained for the cleavage of bigET by α-chymotrypsin (taken as a model proteinase) provides evidence of the potential of SDRPs. This is a novel application of SDRPs used as inhibitors of a proteolytic reaction.
KW - big-Endothelin
KW - Complementary peptide
KW - Endothelin biosynthesis
KW - Substrate depletion
UR - http://www.scopus.com/inward/record.url?scp=0027910612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027910612&partnerID=8YFLogxK
U2 - 10.1016/0167-4838(93)90025-M
DO - 10.1016/0167-4838(93)90025-M
M3 - Article
C2 - 8399398
AN - SCOPUS:0027910612
VL - 1202
SP - 337
EP - 340
JO - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
JF - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
SN - 0167-4838
IS - 2
ER -