Sequence-directed recognition peptides: inhibition of endothelin generation via a substrate-depletion mechanism

Moreno Zamai, Valeria R. Caiolfa

Research output: Contribution to journalArticlepeer-review

Abstract

Sequence-directed recognition peptides (SDRPs) were constructed on the basis of their hydropathic complementarity for big-endothelin (bigET). These peptides can inhibit in vitro the proteolytic cleavage that generates endothelin (ET) from its bigET precursor. Comparison of dissociation constants of the complexes SDRP:bigET with kinetic constants obtained for the cleavage of bigET by α-chymotrypsin (taken as a model proteinase) provides evidence of the potential of SDRPs. This is a novel application of SDRPs used as inhibitors of a proteolytic reaction.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1202
Issue number2
DOIs
Publication statusPublished - Oct 6 1993

Keywords

  • big-Endothelin
  • Complementary peptide
  • Endothelin biosynthesis
  • Substrate depletion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

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