Abstract
Sequence-directed recognition peptides (SDRPs) were constructed on the basis of their hydropathic complementarity for big-endothelin (bigET). These peptides can inhibit in vitro the proteolytic cleavage that generates endothelin (ET) from its bigET precursor. Comparison of dissociation constants of the complexes SDRP:bigET with kinetic constants obtained for the cleavage of bigET by α-chymotrypsin (taken as a model proteinase) provides evidence of the potential of SDRPs. This is a novel application of SDRPs used as inhibitors of a proteolytic reaction.
Original language | English |
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Pages (from-to) | 337-340 |
Number of pages | 4 |
Journal | Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular |
Volume | 1202 |
Issue number | 2 |
DOIs | |
Publication status | Published - Oct 6 1993 |
Keywords
- big-Endothelin
- Complementary peptide
- Endothelin biosynthesis
- Substrate depletion
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Structural Biology
- Medicine(all)