Sequence of a cDNA encoding the β4 subunit of murine integrin

Stephen J. Kennel, Linda J. Foote, Letizia Cimino, Maria Giulia Rizzo, Lan Yang Chang, Ada Sacchi

Research output: Contribution to journalArticlepeer-review

Abstract

A cDNA coding for the β4 subunit of murine integrin (mβ4) has been cloned and sequenced using mRNA from a murine lung carcinoma as the template. The 5' sequence contains two AUG codons, the second of which initiates synthesis of the mature protein. The cDNA sequence has an open reading frame coding for 1748 amino acids (aa), including a signal peptide, cysteine-rich region, serine- and threonine-rich region, transmembrane domain, and a cytoplasmic domain of over 1000 aa. Overall, the deduced mβ4 aa sequence has 88% identity with the human β4 subunit (hβ4) sequence deduced from the sequence of placental mRNA. Reverse transcriptase-polymerase chain reaction using primers flanking splice sites for two variant forms of hβ4 transcripts provided evidence for alternate splicing of RNA in the murine spleen and to a lesser extent in the skin, uterus, and thymus but was found at only one of the two alternative sites. Five potential glycosylation sites present in the extracellular domain of hβ4 are conserved in mβ4. One tyrosine in the terminal region of the cytoplasmic domain (position 1600) is conserved between mβ4 and hβ4 and has the consensus sequence for tyrosine phosphorylation. Finally, a genomic restriction map of mβ4 shows that the gene is about 40 kb in length. No restriction-fragment length polymorphisms were detected between BALB/c liver and BALB/c lung carcinoma DNA.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalGene
Volume130
Issue number2
DOIs
Publication statusPublished - Aug 25 1993

Keywords

  • gene map
  • Lung carcinoma
  • splice variants

ASJC Scopus subject areas

  • Genetics

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