Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase

Massimo Zeviani, Saburo Sakoda, Ariel A. Sherbany, Hirofumi Nakase, Rosario Rizzuto, Craig E. Samitt, Salvatore DiMauro, Eric A. Schon

Research output: Contribution to journalArticlepeer-review


We have isolated a full-length human fetal muscle cDNA clone specifying the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme, cytochrome c oxidase (COX; EC, and a partial-length brain cDNA clone specifying the analogous bovine subunit. The two cDNAs are 85% identical at the nucleotide level. Similar to other proteins imported into mitochondria, the deduced human COX Vb protein contains a presequence, 31 amino acids long, rich in basic residues. We find no evidence for tissue-specific transcripts for subunit Vb of human COX, as Northern analysis of total RNA from human muscle, liver, and brain showed a single, identically sized transcript in each cell type, while partial-length cDNA clones isolated from human muscle and endothelial cell cDNA libraries were identical in sequence to the fetal muscle cDNA.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
Issue number1
Publication statusPublished - May 15 1988


  • 2 and M 13 phage vectors
  • brain
  • electron transport
  • liver
  • Mitochondrial protein
  • muscle
  • nuclear gene
  • nucleotide sequence analysis
  • recombinant DNA
  • respiratory chain

ASJC Scopus subject areas

  • Genetics


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