Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis

Paolo Mariottini, Claudia Bagni, Anna Francesconi, Francesco Cecconi, Martin J. Serra, Qi Min Chen, Fabrizio Loreni, Flavia Annesi, Francesco Amaldi

Research output: Contribution to journalArticle

Abstract

We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalGene
Volume132
Issue number2
DOIs
Publication statusPublished - Oct 15 1993

    Fingerprint

Keywords

  • housekeeping gene
  • Recombinant DNA
  • repeated sequences
  • ribosome

ASJC Scopus subject areas

  • Genetics

Cite this

Mariottini, P., Bagni, C., Francesconi, A., Cecconi, F., Serra, M. J., Chen, Q. M., Loreni, F., Annesi, F., & Amaldi, F. (1993). Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis. Gene, 132(2), 255-260. https://doi.org/10.1016/0378-1119(93)90204-G