Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis

Paolo Mariottini; Claudia Bagni; Anna Francesconi; Francesco Cecconi; Martin J. Serra; Qi Min Chen; Fabrizio Loreni; Flavia Annesi; Francesco Amaldi

doi:10.1016/0378-1119(93)90204-G

Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis

Paolo Mariottini, Claudia Bagni, Anna Francesconi, Francesco Cecconi, Martin J. Serra, Qi Min Chen, Fabrizio Loreni, Flavia Annesi, Francesco Amaldi

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	Gene
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(93)90204-G
Publication status	Published - Oct 15 1993

Keywords

housekeeping gene
Recombinant DNA
repeated sequences
ribosome

ASJC Scopus subject areas

Genetics

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title = "Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis",

abstract = "We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.",

keywords = "housekeeping gene, Recombinant DNA, repeated sequences, ribosome",

author = "Paolo Mariottini and Claudia Bagni and Anna Francesconi and Francesco Cecconi and Serra, {Martin J.} and Chen, {Qi Min} and Fabrizio Loreni and Flavia Annesi and Francesco Amaldi",

year = "1993",

month = oct,

day = "15",

doi = "10.1016/0378-1119(93)90204-G",

language = "English",

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AU - Mariottini, Paolo

AU - Bagni, Claudia

AU - Francesconi, Anna

AU - Cecconi, Francesco

AU - Serra, Martin J.

AU - Chen, Qi Min

AU - Loreni, Fabrizio

AU - Annesi, Flavia

AU - Amaldi, Francesco

PY - 1993/10/15

Y1 - 1993/10/15

N2 - We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

AB - We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

KW - housekeeping gene

KW - Recombinant DNA

KW - repeated sequences

KW - ribosome

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Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis

Abstract

Keywords

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