Abstract
We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
| Original language | English |
|---|---|
| Pages (from-to) | 255-260 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 132 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 15 1993 |
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Keywords
- housekeeping gene
- Recombinant DNA
- repeated sequences
- ribosome
ASJC Scopus subject areas
- Genetics
Cite this
Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis. / Mariottini, Paolo; Bagni, Claudia; Francesconi, Anna; Cecconi, Francesco; Serra, Martin J.; Chen, Qi Min; Loreni, Fabrizio; Annesi, Flavia; Amaldi, Francesco.
In: Gene, Vol. 132, No. 2, 15.10.1993, p. 255-260.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis
AU - Mariottini, Paolo
AU - Bagni, Claudia
AU - Francesconi, Anna
AU - Cecconi, Francesco
AU - Serra, Martin J.
AU - Chen, Qi Min
AU - Loreni, Fabrizio
AU - Annesi, Flavia
AU - Amaldi, Francesco
PY - 1993/10/15
Y1 - 1993/10/15
N2 - We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
AB - We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidinerich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
KW - housekeeping gene
KW - Recombinant DNA
KW - repeated sequences
KW - ribosome
UR - http://www.scopus.com/inward/record.url?scp=0027365587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027365587&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90204-G
DO - 10.1016/0378-1119(93)90204-G
M3 - Article
C2 - 8224872
AN - SCOPUS:0027365587
VL - 132
SP - 255
EP - 260
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -