Sequence organization of the ribosomal spacer of D.melanogaster

A. Simeone, A. De Falco, G. Macino, E. Boncinelli

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We subcloned several fragments of the rDNA nontranscribed spacer (NTS) of D.melanogaster and analysed their nucleotide sequence. The central portion contains a variable number of 240 base pair repeated sequences ending with two contiguous Alu I sites. Within every repeat there is a 42 base pair sequence perfectly homologous to the transcription initiation site from -18 to +24. Sequences homologous to the basic 240 bp repeat are present both at the end of the unique 1900 bp region downstream to the transcription termination site and within the unique region preceeding the transcription initiation site up to nucleotide -140. These results are discussed in relation to the general problem of ribosomal gene units apd their evolution.

Original languageEnglish
Pages (from-to)8263-8272
Number of pages10
JournalNucleic Acids Research
Volume10
Issue number24
DOIs
Publication statusPublished - Dec 20 1982

Fingerprint

Transcription Initiation Site
Transcription
Sequence Homology
Base Pairing
Nucleotides
Ribosomal DNA
Genes
Termination
Fragment
Gene
Unit

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Simeone, A., Falco, A. D., Macino, G., & Boncinelli, E. (1982). Sequence organization of the ribosomal spacer of D.melanogaster. Nucleic Acids Research, 10(24), 8263-8272. https://doi.org/10.1093/nar/10.24.8263

Sequence organization of the ribosomal spacer of D.melanogaster. / Simeone, A.; Falco, A. De; Macino, G.; Boncinelli, E.

In: Nucleic Acids Research, Vol. 10, No. 24, 20.12.1982, p. 8263-8272.

Research output: Contribution to journalArticle

Simeone, A, Falco, AD, Macino, G & Boncinelli, E 1982, 'Sequence organization of the ribosomal spacer of D.melanogaster', Nucleic Acids Research, vol. 10, no. 24, pp. 8263-8272. https://doi.org/10.1093/nar/10.24.8263
Simeone, A. ; Falco, A. De ; Macino, G. ; Boncinelli, E. / Sequence organization of the ribosomal spacer of D.melanogaster. In: Nucleic Acids Research. 1982 ; Vol. 10, No. 24. pp. 8263-8272.
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