Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes

Giordana Feriotto, Carlo Mischiati, Nicoletta Bianchi, Cristina Rutigliano, Patrizio Giacomini, Roberto Gambari

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this paper we report the characterization of a newly sequenced 5′ upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5′ upstream sequences of the murine EA gene, (iv) DNase I footprinting experiments and (v) electrophoretic mobility shift assays. Our results demonstrate the existence, in the HLA-DRA gene, of Y′ and X′ boxes highly homologous to the Y and X boxes present in MHC class II genes, but oriented in the opposite direction. These Y′ and X′ boxes have been conserved during the molecular evolution of both human HLA-DRA and murine EA genes. DNase I footprinting and gel retardation experiments suggest that the X′ and Y′ boxes of the HLA-DRA upstream gene region are specifically recognized by nuclear proteins that also bind to the X and Y boxes of the HLA-DRA proximal promoter, respectively.

Original languageEnglish
Pages (from-to)1671-1678
Number of pages8
JournalNucleic Acids Research
Volume23
Issue number10
Publication statusPublished - May 25 1995

Fingerprint

HLA-DR alpha-Chains
Sequencing
Genes
Gene
Deoxyribonuclease I
MHC Class II Genes
Molecular Evolution
Electrophoretic Mobility Shift Assay
Electrophoretic mobility
Nuclear Proteins
Transcription factors
Transcription Factor
Promoter
Transcription Factors
Gels
Experiment
Human
Assays
Display
Experiments

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Feriotto, G., Mischiati, C., Bianchi, N., Rutigliano, C., Giacomini, P., & Gambari, R. (1995). Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes. Nucleic Acids Research, 23(10), 1671-1678.

Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes. / Feriotto, Giordana; Mischiati, Carlo; Bianchi, Nicoletta; Rutigliano, Cristina; Giacomini, Patrizio; Gambari, Roberto.

In: Nucleic Acids Research, Vol. 23, No. 10, 25.05.1995, p. 1671-1678.

Research output: Contribution to journalArticle

Feriotto, G, Mischiati, C, Bianchi, N, Rutigliano, C, Giacomini, P & Gambari, R 1995, 'Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes', Nucleic Acids Research, vol. 23, no. 10, pp. 1671-1678.
Feriotto G, Mischiati C, Bianchi N, Rutigliano C, Giacomini P, Gambari R. Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes. Nucleic Acids Research. 1995 May 25;23(10):1671-1678.
Feriotto, Giordana ; Mischiati, Carlo ; Bianchi, Nicoletta ; Rutigliano, Cristina ; Giacomini, Patrizio ; Gambari, Roberto. / Sequencing of an upstream region of the human HLA-DRA gene containing X′ and Y′ boxes. In: Nucleic Acids Research. 1995 ; Vol. 23, No. 10. pp. 1671-1678.
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