Sequential waves of functionally related proteins are expressed when B cells prepare for antibody secretion

Eelco Van Anken, Edwin P. Romijn, Claudia Maggioni, Alexandre Mezghrani, Roberto Sitia, Ineke Braakman, Albert J R Heck

Research output: Contribution to journalArticlepeer-review

Abstract

Upon encounter with antigen, B lymphocytes differentiate into Ig-secreting plasma cells. This step involves a massive development of secretory organelles, most notably the endoplasmic reticulum. To analyze the relationship between organelle reshaping and Ig secretion, we performed a dynamic proteomics study of B lymphoma cells undergoing in vitro terminal differentiation. By clustering proteins according to temporal expression patterns, it appeared that B cells anticipate their secretory role in a multistep process. Metabolic capacity and secretory machinery expand first to accommodate the mass production of IgM that follows.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalImmunity
Volume18
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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