Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells

Jamil Azzi, Nikolaos Skartsis, Marwan Mounayar, Ciara N. Magee, Ibrahim Batal, Christopher Ting, Robert Moore, Leonardo V. Riella, Shunsuke Ohori, Rozita Abdoli, Brian Smith, Paolo Fiorina, Dean Heathcote, Tannous Bakhos, Philip G. Ashton-Rickardt, Reza Abdi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Regulatory T cells (Tregs) play a pivotal role in the maintenance of immune tolerance and hold great promise as cell therapy for a variety of immune-mediated diseases. However, the cellular mechanisms that regulate Treg maintenance and homeostasis have yet to be fully explored. Although Tregs express granzyme-B (GrB) to suppress effector T cells via direct killing, the mechanisms by which they protect themselves from GrB-mediated self-inflicted damage are unknown. To our knowledge, we show for the first time that both induced Tregs and natural Tregs (nTregs) increase their intracellular expression of GrB and its endogenous inhibitor, serine protease inhibitor 6 (Spi6) upon activation. Subcellular fractionation and measurement of GrB activity in the cytoplasm of Tregs show that activated Spi6-/- Tregs had significantly higher cytoplasmic GrB activity. We observed an increase in GrBmediated apoptosis in Spi6-/- nTregs and impaired suppression of alloreactive T cells in vitro. Spi6-/- Tregs were rescued from apoptosis by the addition of a GrB inhibitor (Z-AAD-CMK) in vitro. Furthermore, adoptive transfer experiments showed that Spi6-/- nTregs were less effective than wild type nTregs in suppressing graft-versus-host disease because of their impaired survival, as shown in our in vivo bioluminescence imaging. Finally, Spi6-deficient recipients rejected MHC class II-mismatch heart allografts at a much faster rate and showed a higher rate of apoptosis among Tregs, as compared with wild type recipients. To our knowledge, our data demonstrate, for the first time, a novel role for Spi6 in Treg homeostasis by protecting activated Tregs from GrB-mediated injury. These data could have significant clinical implications for Treg-based therapy in immune-mediated diseases.

Original languageEnglish
Pages (from-to)2319-2327
Number of pages9
JournalJournal of Immunology
Volume191
Issue number5
DOIs
Publication statusPublished - Sep 1 2013

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Granzymes
Serine Proteinase Inhibitors
Regulatory T-Lymphocytes
Immune System Diseases
Apoptosis
Homeostasis
Maintenance
T-Lymphocytes
Immune Tolerance
Adoptive Transfer
Population Growth
Graft vs Host Disease
Cell- and Tissue-Based Therapy
Allografts
Cytoplasm
Wounds and Injuries

ASJC Scopus subject areas

  • Immunology

Cite this

Azzi, J., Skartsis, N., Mounayar, M., Magee, C. N., Batal, I., Ting, C., ... Abdi, R. (2013). Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells. Journal of Immunology, 191(5), 2319-2327. https://doi.org/10.4049/jimmunol.1300851

Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells. / Azzi, Jamil; Skartsis, Nikolaos; Mounayar, Marwan; Magee, Ciara N.; Batal, Ibrahim; Ting, Christopher; Moore, Robert; Riella, Leonardo V.; Ohori, Shunsuke; Abdoli, Rozita; Smith, Brian; Fiorina, Paolo; Heathcote, Dean; Bakhos, Tannous; Ashton-Rickardt, Philip G.; Abdi, Reza.

In: Journal of Immunology, Vol. 191, No. 5, 01.09.2013, p. 2319-2327.

Research output: Contribution to journalArticle

Azzi, J, Skartsis, N, Mounayar, M, Magee, CN, Batal, I, Ting, C, Moore, R, Riella, LV, Ohori, S, Abdoli, R, Smith, B, Fiorina, P, Heathcote, D, Bakhos, T, Ashton-Rickardt, PG & Abdi, R 2013, 'Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells', Journal of Immunology, vol. 191, no. 5, pp. 2319-2327. https://doi.org/10.4049/jimmunol.1300851
Azzi, Jamil ; Skartsis, Nikolaos ; Mounayar, Marwan ; Magee, Ciara N. ; Batal, Ibrahim ; Ting, Christopher ; Moore, Robert ; Riella, Leonardo V. ; Ohori, Shunsuke ; Abdoli, Rozita ; Smith, Brian ; Fiorina, Paolo ; Heathcote, Dean ; Bakhos, Tannous ; Ashton-Rickardt, Philip G. ; Abdi, Reza. / Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells. In: Journal of Immunology. 2013 ; Vol. 191, No. 5. pp. 2319-2327.
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AU - Moore, Robert

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AU - Abdoli, Rozita

AU - Smith, Brian

AU - Fiorina, Paolo

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