Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles

Irene Melegari, Fulvio Marsilio, Federica Di Profio, Vittorio Sarchese, Ivano Massirio, Andrea Palombieri, Anna Rita D'Angelo, Gianvito Lanave, Georgia Diakoudi, Alessandra Cavalli, Vito Martella, Barbara Di Martino

Research output: Contribution to journalArticlepeer-review


Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0–2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalVirus Research
Publication statusPublished - Jun 2 2018


  • Canine sapoviruses
  • Dogs
  • IgG antibodies
  • Virus like-particles

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

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