Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles

Irene Melegari, Fulvio Marsilio, Federica Di Profio, Vittorio Sarchese, Ivano Massirio, Andrea Palombieri, Anna Rita D'Angelo, Gianvito Lanave, Georgia Diakoudi, Alessandra Cavalli, Vito Martella, Barbara Di Martino

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Abstract

Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0–2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalVirus Research
Volume251
DOIs
Publication statusPublished - Jun 2 2018

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Keywords

  • Canine sapoviruses
  • Dogs
  • IgG antibodies
  • Virus like-particles

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Melegari, I., Marsilio, F., Di Profio, F., Sarchese, V., Massirio, I., Palombieri, A., D'Angelo, A. R., Lanave, G., Diakoudi, G., Cavalli, A., Martella, V., & Di Martino, B. (2018). Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. Virus Research, 251, 1-5. https://doi.org/10.1016/j.virusres.2018.04.014