Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles

Irene Melegari, Fulvio Marsilio, Federica Di Profio, Vittorio Sarchese, Ivano Massirio, Andrea Palombieri, Anna Rita D'Angelo, Gianvito Lanave, Georgia Diakoudi, Alessandra Cavalli, Vito Martella, Barbara Di Martino

Research output: Contribution to journalArticle

Abstract

Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0–2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalVirus Research
Volume251
DOIs
Publication statusPublished - Jun 2 2018

Fingerprint

Sapovirus
Baculoviridae
Seroepidemiologic Studies
Virion
Dogs
Canidae
Serum
Italy
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Viruses
Antigens
Antibodies
Infection

Keywords

  • Canine sapoviruses
  • Dogs
  • IgG antibodies
  • Virus like-particles

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Melegari, I., Marsilio, F., Di Profio, F., Sarchese, V., Massirio, I., Palombieri, A., ... Di Martino, B. (2018). Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. Virus Research, 251, 1-5. https://doi.org/10.1016/j.virusres.2018.04.014

Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. / Melegari, Irene; Marsilio, Fulvio; Di Profio, Federica; Sarchese, Vittorio; Massirio, Ivano; Palombieri, Andrea; D'Angelo, Anna Rita; Lanave, Gianvito; Diakoudi, Georgia; Cavalli, Alessandra; Martella, Vito; Di Martino, Barbara.

In: Virus Research, Vol. 251, 02.06.2018, p. 1-5.

Research output: Contribution to journalArticle

Melegari, I, Marsilio, F, Di Profio, F, Sarchese, V, Massirio, I, Palombieri, A, D'Angelo, AR, Lanave, G, Diakoudi, G, Cavalli, A, Martella, V & Di Martino, B 2018, 'Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles', Virus Research, vol. 251, pp. 1-5. https://doi.org/10.1016/j.virusres.2018.04.014
Melegari I, Marsilio F, Di Profio F, Sarchese V, Massirio I, Palombieri A et al. Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. Virus Research. 2018 Jun 2;251:1-5. https://doi.org/10.1016/j.virusres.2018.04.014
Melegari, Irene ; Marsilio, Fulvio ; Di Profio, Federica ; Sarchese, Vittorio ; Massirio, Ivano ; Palombieri, Andrea ; D'Angelo, Anna Rita ; Lanave, Gianvito ; Diakoudi, Georgia ; Cavalli, Alessandra ; Martella, Vito ; Di Martino, Barbara. / Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. In: Virus Research. 2018 ; Vol. 251. pp. 1-5.
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