Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era

Gianvincenzo Zuccotti; Chiara Mameli; Laura Daprai; Maria Laura Garlaschi; Dario Dilillo; Giorgio Bedogni; Marino Faccini; Maria Gramegna; Erminio Torresani; Ballerini Emanuela; Benincaso Annarita; Bonvissuto Milena; Bricalli Dorella; Brioschi Manuela; Calloni Cinzia Simona; Camiletti Marina Irene; Colella Giacomo; De Angelis Laura; Decarlis Silvia; Di Nello Francesca; Dozzi Massimiliano; Galli Erica; Gandini Vera; Giuliani Maria Grazia; Laviola Franca; Loda Barbara; Macedoni Maddalena; Mazzucchi Elisabetta; Metta Maria Gabriella; Moscatiello Anna; Nannini Pilar; Petruzzi Mariangela; Picicco Damiano; Picciotti Michela; Pisanelli Stefania; Porta Norberto; Ramponi Giulia; Redaelli Francesca; Rubini Riccardo; Sala Natascia; Saitta Vincenzo; Scelza Giuseppina; Tiso Rosa Maria; Tomasetto Mariangela; Torcoletti Matteo; Travaini Marta; Valentini Maurizio; Vessia Chiara

doi:10.1016/j.vaccine.2013.12.003

Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era

Gianvincenzo Zuccotti, Chiara Mameli, Laura Daprai, Maria Laura Garlaschi, Dario Dilillo, Giorgio Bedogni, Marino Faccini, Maria Gramegna, Erminio Torresani, Ballerini Emanuela, Benincaso Annarita, Bonvissuto Milena, Bricalli Dorella, Brioschi Manuela, Calloni Cinzia Simona, Camiletti Marina Irene, Colella Giacomo, De Angelis Laura, Decarlis Silvia, Di Nello FrancescaDozzi Massimiliano, Galli Erica, Gandini Vera, Giuliani Maria Grazia, Laviola Franca, Loda Barbara, Macedoni Maddalena, Mazzucchi Elisabetta, Metta Maria Gabriella, Moscatiello Anna, Nannini Pilar, Petruzzi Mariangela, Picicco Damiano, Picciotti Michela, Pisanelli Stefania, Porta Norberto, Ramponi Giulia, Redaelli Francesca, Rubini Riccardo, Sala Natascia, Saitta Vincenzo, Scelza Giuseppina, Tiso Rosa Maria, Tomasetto Mariangela, Torcoletti Matteo, Travaini Marta, Valentini Maurizio, Vessia Chiara

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

Few epidemiological data are available since the introduction of 13-valent pneumococcal vaccine (PCV13) in 2010. We conducted a cross-sectional study to estimate the prevalence of Streptococcus pneumoniae (SP) nasopharyngeal carriage in healthy Italian infants and young children and to evaluate the impact of PCV13 on pneumococcal colonization. In the trimester September-December 2011 nasopharyngeal swabs were collected from healthy children aged 3-59 months presenting for routine well careat 16 primary care pediatricians in Milan.SP carriage isolates were serotyped and tested for antimicrobial resistance using EUCAST breakpoints. Among 1250 enrolled children, 618 had received at least 1 dose of PCV13, 292 at least 1 dose of PCV7, 94 a combination of the two vaccines and 246 were not vaccinated. The prevalence of SP carriage was 27% (95% confidence interval [CI] 25-30).At multivariable analysis, age. ≥. 25 months (prevalence ratio [PR]. = 0.74) and use of antibiotics in the previous 3 months (PR. = 0.67) were associated with lower SP carriage prevalence. Having siblings (PR. = 1.79 for 1 sibling and PR. = 2.23 for ≥2 siblings), day-care attendance (PR. = 2.27) and respiratory tract infections in the previous 3 months (PR. = 1.39) were associated with higher SP carriage prevalence.The immunization status for SP was not associated with SP carriage at univariable or at multivariable analysis.The most common carriage isolates were 6C, 19A and 23A. The prevalence of the six additional PCV13 serotypes carriage in children appropriately vaccinated with PCV13 was lower than in children appropriately vaccinated with PCV7 (0 vs. 0.060); the greater reduction in prevalence of carriage was observed for serotype 19A (0 vs. 0.041). Serotype 6C was the most common drug-resistant serotype (17.2%).Further epidemiological studies are needed to assess changes in circulating SP serotypes following the large-scale introduction of PCV13.

Original language	English
Pages (from-to)	527-534
Number of pages	8
Journal	Vaccine
Volume	32
Issue number	5
DOIs	https://doi.org/10.1016/j.vaccine.2013.12.003
Publication status	Published - Jan 23 2014

Keywords

13-Valent pneumococcal conjugate vaccine
Children
Pneumococcal nasopharyngeal carriage
Streptococcus pneumoniae

ASJC Scopus subject areas

Immunology and Microbiology(all)
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)
Molecular Medicine

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Zuccotti, G., Mameli, C., Daprai, L., Garlaschi, M. L., Dilillo, D., Bedogni, G., Faccini, M., Gramegna, M., Torresani, E., Emanuela, B., Annarita, B., Milena, B., Dorella, B., Manuela, B., Simona, C. C., Irene, C. M., Giacomo, C., Laura, D. A., Silvia, D., ... Chiara, V. (2014). Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era. Vaccine, 32(5), 527-534. https://doi.org/10.1016/j.vaccine.2013.12.003

Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era. / Zuccotti, Gianvincenzo; Mameli, Chiara; Daprai, Laura; Garlaschi, Maria Laura; Dilillo, Dario; Bedogni, Giorgio; Faccini, Marino; Gramegna, Maria; Torresani, Erminio; Emanuela, Ballerini; Annarita, Benincaso; Milena, Bonvissuto; Dorella, Bricalli; Manuela, Brioschi; Simona, Calloni Cinzia; Irene, Camiletti Marina; Giacomo, Colella; Laura, De Angelis; Silvia, Decarlis; Francesca, Di Nello; Massimiliano, Dozzi; Erica, Galli; Vera, Gandini; Grazia, Giuliani Maria; Franca, Laviola; Barbara, Loda; Maddalena, Macedoni; Elisabetta, Mazzucchi; Gabriella, Metta Maria; Anna, Moscatiello; Pilar, Nannini; Mariangela, Petruzzi; Damiano, Picicco; Michela, Picciotti; Stefania, Pisanelli; Norberto, Porta; Giulia, Ramponi; Francesca, Redaelli; Riccardo, Rubini; Natascia, Sala; Vincenzo, Saitta; Giuseppina, Scelza; Maria, Tiso Rosa; Mariangela, Tomasetto; Matteo, Torcoletti; Marta, Travaini; Maurizio, Valentini; Chiara, Vessia.

In: Vaccine, Vol. 32, No. 5, 23.01.2014, p. 527-534.

Research output: Contribution to journal › Article › peer-review

Zuccotti, G, Mameli, C, Daprai, L, Garlaschi, ML, Dilillo, D, Bedogni, G, Faccini, M, Gramegna, M, Torresani, E, Emanuela, B, Annarita, B, Milena, B, Dorella, B, Manuela, B, Simona, CC, Irene, CM, Giacomo, C, Laura, DA, Silvia, D, Francesca, DN, Massimiliano, D, Erica, G, Vera, G, Grazia, GM, Franca, L, Barbara, L, Maddalena, M, Elisabetta, M, Gabriella, MM, Anna, M, Pilar, N, Mariangela, P, Damiano, P, Michela, P, Stefania, P, Norberto, P, Giulia, R, Francesca, R, Riccardo, R, Natascia, S, Vincenzo, S, Giuseppina, S, Maria, TR, Mariangela, T, Matteo, T, Marta, T, Maurizio, V & Chiara, V 2014, 'Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era', Vaccine, vol. 32, no. 5, pp. 527-534. https://doi.org/10.1016/j.vaccine.2013.12.003

Zuccotti, Gianvincenzo ; Mameli, Chiara ; Daprai, Laura ; Garlaschi, Maria Laura ; Dilillo, Dario ; Bedogni, Giorgio ; Faccini, Marino ; Gramegna, Maria ; Torresani, Erminio ; Emanuela, Ballerini ; Annarita, Benincaso ; Milena, Bonvissuto ; Dorella, Bricalli ; Manuela, Brioschi ; Simona, Calloni Cinzia ; Irene, Camiletti Marina ; Giacomo, Colella ; Laura, De Angelis ; Silvia, Decarlis ; Francesca, Di Nello ; Massimiliano, Dozzi ; Erica, Galli ; Vera, Gandini ; Grazia, Giuliani Maria ; Franca, Laviola ; Barbara, Loda ; Maddalena, Macedoni ; Elisabetta, Mazzucchi ; Gabriella, Metta Maria ; Anna, Moscatiello ; Pilar, Nannini ; Mariangela, Petruzzi ; Damiano, Picicco ; Michela, Picciotti ; Stefania, Pisanelli ; Norberto, Porta ; Giulia, Ramponi ; Francesca, Redaelli ; Riccardo, Rubini ; Natascia, Sala ; Vincenzo, Saitta ; Giuseppina, Scelza ; Maria, Tiso Rosa ; Mariangela, Tomasetto ; Matteo, Torcoletti ; Marta, Travaini ; Maurizio, Valentini ; Chiara, Vessia. / Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era. In: Vaccine. 2014 ; Vol. 32, No. 5. pp. 527-534.

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title = "Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era",

abstract = "Few epidemiological data are available since the introduction of 13-valent pneumococcal vaccine (PCV13) in 2010. We conducted a cross-sectional study to estimate the prevalence of Streptococcus pneumoniae (SP) nasopharyngeal carriage in healthy Italian infants and young children and to evaluate the impact of PCV13 on pneumococcal colonization. In the trimester September-December 2011 nasopharyngeal swabs were collected from healthy children aged 3-59 months presenting for routine well careat 16 primary care pediatricians in Milan.SP carriage isolates were serotyped and tested for antimicrobial resistance using EUCAST breakpoints. Among 1250 enrolled children, 618 had received at least 1 dose of PCV13, 292 at least 1 dose of PCV7, 94 a combination of the two vaccines and 246 were not vaccinated. The prevalence of SP carriage was 27% (95% confidence interval [CI] 25-30).At multivariable analysis, age. ≥. 25 months (prevalence ratio [PR]. = 0.74) and use of antibiotics in the previous 3 months (PR. = 0.67) were associated with lower SP carriage prevalence. Having siblings (PR. = 1.79 for 1 sibling and PR. = 2.23 for ≥2 siblings), day-care attendance (PR. = 2.27) and respiratory tract infections in the previous 3 months (PR. = 1.39) were associated with higher SP carriage prevalence.The immunization status for SP was not associated with SP carriage at univariable or at multivariable analysis.The most common carriage isolates were 6C, 19A and 23A. The prevalence of the six additional PCV13 serotypes carriage in children appropriately vaccinated with PCV13 was lower than in children appropriately vaccinated with PCV7 (0 vs. 0.060); the greater reduction in prevalence of carriage was observed for serotype 19A (0 vs. 0.041). Serotype 6C was the most common drug-resistant serotype (17.2%).Further epidemiological studies are needed to assess changes in circulating SP serotypes following the large-scale introduction of PCV13.",

keywords = "13-Valent pneumococcal conjugate vaccine, Children, Pneumococcal nasopharyngeal carriage, Streptococcus pneumoniae",

author = "Gianvincenzo Zuccotti and Chiara Mameli and Laura Daprai and Garlaschi, {Maria Laura} and Dario Dilillo and Giorgio Bedogni and Marino Faccini and Maria Gramegna and Erminio Torresani and Ballerini Emanuela and Benincaso Annarita and Bonvissuto Milena and Bricalli Dorella and Brioschi Manuela and Simona, {Calloni Cinzia} and Irene, {Camiletti Marina} and Colella Giacomo and Laura, {De Angelis} and Decarlis Silvia and Francesca, {Di Nello} and Dozzi Massimiliano and Galli Erica and Gandini Vera and Grazia, {Giuliani Maria} and Laviola Franca and Loda Barbara and Macedoni Maddalena and Mazzucchi Elisabetta and Gabriella, {Metta Maria} and Moscatiello Anna and Nannini Pilar and Petruzzi Mariangela and Picicco Damiano and Picciotti Michela and Pisanelli Stefania and Porta Norberto and Ramponi Giulia and Redaelli Francesca and Rubini Riccardo and Sala Natascia and Saitta Vincenzo and Scelza Giuseppina and Maria, {Tiso Rosa} and Tomasetto Mariangela and Torcoletti Matteo and Travaini Marta and Valentini Maurizio and Vessia Chiara",

year = "2014",

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doi = "10.1016/j.vaccine.2013.12.003",

language = "English",

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T1 - Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from healthy children in the 13-valent pneumococcal conjugate vaccine era

AU - Zuccotti, Gianvincenzo

AU - Mameli, Chiara

AU - Daprai, Laura

AU - Garlaschi, Maria Laura

AU - Dilillo, Dario

AU - Bedogni, Giorgio

AU - Faccini, Marino

AU - Gramegna, Maria

AU - Torresani, Erminio

AU - Emanuela, Ballerini

AU - Annarita, Benincaso

AU - Milena, Bonvissuto

AU - Dorella, Bricalli

AU - Manuela, Brioschi

AU - Simona, Calloni Cinzia

AU - Irene, Camiletti Marina

AU - Giacomo, Colella

AU - Laura, De Angelis

AU - Silvia, Decarlis

AU - Francesca, Di Nello

AU - Massimiliano, Dozzi

AU - Erica, Galli

AU - Vera, Gandini

AU - Grazia, Giuliani Maria

AU - Franca, Laviola

AU - Barbara, Loda

AU - Maddalena, Macedoni

AU - Elisabetta, Mazzucchi

AU - Gabriella, Metta Maria

AU - Anna, Moscatiello

AU - Pilar, Nannini

AU - Mariangela, Petruzzi

AU - Damiano, Picicco

AU - Michela, Picciotti

AU - Stefania, Pisanelli

AU - Norberto, Porta

AU - Giulia, Ramponi

AU - Francesca, Redaelli

AU - Riccardo, Rubini

AU - Natascia, Sala

AU - Vincenzo, Saitta

AU - Giuseppina, Scelza

AU - Maria, Tiso Rosa

AU - Mariangela, Tomasetto

AU - Matteo, Torcoletti

AU - Marta, Travaini

AU - Maurizio, Valentini

AU - Chiara, Vessia

PY - 2014/1/23

Y1 - 2014/1/23

N2 - Few epidemiological data are available since the introduction of 13-valent pneumococcal vaccine (PCV13) in 2010. We conducted a cross-sectional study to estimate the prevalence of Streptococcus pneumoniae (SP) nasopharyngeal carriage in healthy Italian infants and young children and to evaluate the impact of PCV13 on pneumococcal colonization. In the trimester September-December 2011 nasopharyngeal swabs were collected from healthy children aged 3-59 months presenting for routine well careat 16 primary care pediatricians in Milan.SP carriage isolates were serotyped and tested for antimicrobial resistance using EUCAST breakpoints. Among 1250 enrolled children, 618 had received at least 1 dose of PCV13, 292 at least 1 dose of PCV7, 94 a combination of the two vaccines and 246 were not vaccinated. The prevalence of SP carriage was 27% (95% confidence interval [CI] 25-30).At multivariable analysis, age. ≥. 25 months (prevalence ratio [PR]. = 0.74) and use of antibiotics in the previous 3 months (PR. = 0.67) were associated with lower SP carriage prevalence. Having siblings (PR. = 1.79 for 1 sibling and PR. = 2.23 for ≥2 siblings), day-care attendance (PR. = 2.27) and respiratory tract infections in the previous 3 months (PR. = 1.39) were associated with higher SP carriage prevalence.The immunization status for SP was not associated with SP carriage at univariable or at multivariable analysis.The most common carriage isolates were 6C, 19A and 23A. The prevalence of the six additional PCV13 serotypes carriage in children appropriately vaccinated with PCV13 was lower than in children appropriately vaccinated with PCV7 (0 vs. 0.060); the greater reduction in prevalence of carriage was observed for serotype 19A (0 vs. 0.041). Serotype 6C was the most common drug-resistant serotype (17.2%).Further epidemiological studies are needed to assess changes in circulating SP serotypes following the large-scale introduction of PCV13.

AB - Few epidemiological data are available since the introduction of 13-valent pneumococcal vaccine (PCV13) in 2010. We conducted a cross-sectional study to estimate the prevalence of Streptococcus pneumoniae (SP) nasopharyngeal carriage in healthy Italian infants and young children and to evaluate the impact of PCV13 on pneumococcal colonization. In the trimester September-December 2011 nasopharyngeal swabs were collected from healthy children aged 3-59 months presenting for routine well careat 16 primary care pediatricians in Milan.SP carriage isolates were serotyped and tested for antimicrobial resistance using EUCAST breakpoints. Among 1250 enrolled children, 618 had received at least 1 dose of PCV13, 292 at least 1 dose of PCV7, 94 a combination of the two vaccines and 246 were not vaccinated. The prevalence of SP carriage was 27% (95% confidence interval [CI] 25-30).At multivariable analysis, age. ≥. 25 months (prevalence ratio [PR]. = 0.74) and use of antibiotics in the previous 3 months (PR. = 0.67) were associated with lower SP carriage prevalence. Having siblings (PR. = 1.79 for 1 sibling and PR. = 2.23 for ≥2 siblings), day-care attendance (PR. = 2.27) and respiratory tract infections in the previous 3 months (PR. = 1.39) were associated with higher SP carriage prevalence.The immunization status for SP was not associated with SP carriage at univariable or at multivariable analysis.The most common carriage isolates were 6C, 19A and 23A. The prevalence of the six additional PCV13 serotypes carriage in children appropriately vaccinated with PCV13 was lower than in children appropriately vaccinated with PCV7 (0 vs. 0.060); the greater reduction in prevalence of carriage was observed for serotype 19A (0 vs. 0.041). Serotype 6C was the most common drug-resistant serotype (17.2%).Further epidemiological studies are needed to assess changes in circulating SP serotypes following the large-scale introduction of PCV13.

KW - 13-Valent pneumococcal conjugate vaccine

KW - Children

KW - Pneumococcal nasopharyngeal carriage

KW - Streptococcus pneumoniae

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