SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells

Lucio Litti, Andrea Colusso, Marcella Pinto, Erlis Ruli, Alessia Scarsi, Laura Ventura, Giuseppe Toffoli, Marco Colombatti, Giulio Fracasso, Moreno Meneghetti

Research output: Contribution to journalArticlepeer-review

Abstract

Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new protocol based on surface enhanced resonance Raman scattering (SERRS) gold multivalent nanostructures to identify and enumerate tumor cells with epithelial and mesenchimal markers. The validation of the protocol is obtained with spiked samples of peripheral blood mononuclear cells (PBMC). Gold nanostructures are functionalized with SERRS labels and with antibodies to link the tumor cells. Three types of such nanosystems were simultaneously used and the protocol allows obtaining the identification of all individual tumor cells with the help of a Random Forest ensemble learning method.

Original languageEnglish
Article number15805
Number of pages10
JournalScientific Reports
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2020

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'SERRS multiplexing with multivalent nanostructures for the identification and enumeration of epithelial and mesenchymal cells'. Together they form a unique fingerprint.

Cite this