SERS active Ag nanoparticles in mesoporous silicon: Detection of organic molecules and peptide-antibody assays

Alessandro Virga, Paola Rivolo, Emiliano Descrovi, Alessandro Chiolerio, Gabriella Digregorio, Francesca Frascella, Marco Soster, Federico Bussolino, Serena Marchiò, Francesco Geobaldo, Fabrizio Giorgis

Research output: Contribution to journalArticle

Abstract

Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface-enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV-Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1',3',3'-esamethyl-5,5'- dimethoxyindodicarbocyanine iodide (Cy5-OCH 3) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 10 7. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.

Original languageEnglish
Pages (from-to)730-736
Number of pages7
JournalJournal of Raman Spectroscopy
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 2012

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Keywords

  • cyanine dye
  • peptide-antibody assays
  • porous silicon
  • SERS

ASJC Scopus subject areas

  • Spectroscopy
  • Materials Science(all)

Cite this

Virga, A., Rivolo, P., Descrovi, E., Chiolerio, A., Digregorio, G., Frascella, F., Soster, M., Bussolino, F., Marchiò, S., Geobaldo, F., & Giorgis, F. (2012). SERS active Ag nanoparticles in mesoporous silicon: Detection of organic molecules and peptide-antibody assays. Journal of Raman Spectroscopy, 43(6), 730-736. https://doi.org/10.1002/jrs.3086