Serum 27-hydroxycholesterol in patients with primary biliary cirrhosis suggests alteration of cholesterol catabolism to bile acids via the acidic pathway

M. Del Puppo, M. Galli Kienle, M. L. Petroni, A. Crosignani, M. Podda

Research output: Contribution to journalArticle

Abstract

Reduced cholesterol synthesis has been reported in patients with primary biliary cirrhosis but no data are available on changes in cholesterol catabolism induced by the disease. Serum levels of 7α-hydroxycholesterol and 27-hydroxycholesterol have been measured in 25 patients (either normocholesterolemic or hypercholesterolemic) with primary biliary cirrhosis and in control subjects. To evaluate cholesterol synthesis, serum levels of lathosterol were measured, and campesterol and sitosterol were considered to reflect intestinal absorption and biliary elimination of sterols. In normocholesterolemic patients with primary biliary cirrhosis, lathosterol was significantly lower than in normocholesterolemic controls (P <0.05) whereas no difference was found between hypercholesterolemic patients and hypercholesterolemic controls. Serum concentrations of sitosterol were significantly higher in both normocholesterolemic and hypercholesterolemic patients with primary biliary cirrhosis as compared with the respective controls (P <0.01). In patients with primary biliary cirrhosis, serum 7α- hydroxycholesterol was slightly higher than in controls. 27- Hydroxycholesterol was significantly higher in hypercholesterolemic compared to normocholesterolemic controls (P <0.05) and a significant linear correlation (r = 0.771; P <0.001) was found between 27-hydroxycholesterol and cholesterol. In contrast, in patients with primary biliary cirrhosis, high cholesterol concentrations were not associated with increased serum levels of 27-hydroxycholesterol. Our data confirm that in patients with primary biliary cirrhosis, cholesterol synthesis and biliary elimination of sterois are impaired and also suggest that both the feedback regulation of retained bile acids on cholesterol 7α-hydroxylase and the scavenger effect on elevated serum cholesterol by cholesterol 27-hydroxylase are deficient in these patients.

Original languageEnglish
Pages (from-to)2477-2482
Number of pages6
JournalJournal of Lipid Research
Volume39
Issue number12
Publication statusPublished - 1998

Keywords

  • 27-hydroxycholesterol
  • 7α-hydroxycholesterol
  • Bile acid synthesis
  • Cholesterol metabolism
  • Cholesterol transport
  • Dietary sterols
  • Hypercholesterolemia
  • Lathosterol
  • Mass spectrometry

ASJC Scopus subject areas

  • Endocrinology

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