Serum amyloid A: Production by human white adipocyte and regulation by obesity and nutrition

C. Poitou, N. Viguerie, R. Cancello, R. De Matteis, S. Cinti, V. Stich, C. Coussieu, E. Gauthier, M. Courtine, J. D. Zucker, G. S. Barsh, W. Saris, P. Bruneval, A. Basdevant, D. Langin, K. Clément

Research output: Contribution to journalArticlepeer-review

Abstract

Aims/hypothesis: The acute-phase proteins, serum amyloid As (SAA), are precursors of amyloid A, involved in the pathogenesis of AA amyloidosis. This work started with the characterisation of systemic AA amyloidosis concurrent with SAA overexpression in the subcutaneous white adipose tissue (sWAT) of an obese patient with a leptin receptor deficiency. In the present study a series of histopathological, cellular and gene expression studies was performed to assess the importance of SAA in common obesity and its possible production by mature adipocytes. Materials and methods: Gene expression profiling was performed in the sWAT of two extremely obese patients with a leptin receptor deficiency. Levels of the mRNAs of the different SAA isoforms were quantified in sWAT cellular fractions from lean subjects and from obese subjects before and after a very-low-calorie diet. These values were subsequently compared with serum levels of SAA in these individuals. In addition, histopathological analyses of sWAT were performed in lean and obese subjects. Results: In sWAT, the expression of SAA is more than 20-fold higher in mature adipocytes than in the cells of the stroma vascular fraction (p

Original languageEnglish
Pages (from-to)519-528
Number of pages10
JournalDiabetologia
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2005

Keywords

  • Adipocyte
  • Amyloidosis
  • Calorie restriction
  • Gene expression profiling
  • Leptin receptor
  • Obesity
  • Serum amyloid A
  • Stroma vascular fraction
  • Very-low-calorie diet

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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