Serum and synovial fluid concentrations of matrix metalloproteinases 3 and its tissue inhibitor 1 in juvenile idiopathic arthritides

Marco Gattorno, Silvia Vignola, Fernanda Falcini, Federica Sabatini, Antonella Buoncompagni, Gabriele Simonini, Paolo Picco, Vito Pistoia

Research output: Contribution to journalArticle

Abstract

Objective. Matrix metalloproteinases (MMP) are a large family of proteolytic enzymes involved in the remodeling of extracellular matrix during tissue resorption in idiopathic arthritides. We investigated serum and synovial fluid (SF) concentrations of MMP-3 and its tissue inhibitor (TIMP-1) in juvenile idiopathic arthritides (JIA). Methods. Sera from 45 patients with active, 15 patients with inactive JIA, and 15 healthy controls were evaluated by ELISA for MMP-3 (stromelysin-1), TIMP-1, and soluble p75 tumor necrosis factor receptor (sTNFR). Paired SF concentrations were evaluated in 19 patients with JIA. Results. MMP-3 serum concentrations were significantly higher in patients with active poly- and oligoarticular JIA versus inactive patients (p = 0.04 and p = 0.02, respectively) and healthy controls (p <0.001 for both). Serum MMP-3, but not TIMP-1, concentration displayed a variable degree of correlation with clinical and laboratory variables of disease activity and with p75 sTNFR concentrations (r = 0.37, p = 0.005). SF MMP-3 concentrations were 30-300 times higher than those found in paired sera (p <0.001, Wilcoxon rank test). A clear inversion of MMP-3/TIMP-1 ratio was observed when sera (median 0.31, range 0.02-1.5) were compared with the corresponding SF samples (5.3, range 4.9-5.5; p <0.001). Conclusion. MMP-3 (stromelysin-1) is clearly overexpressed in SF of patients with JIA. An inadequate counter-expression of TIMP-1 may represent a crucial event for the development and perpetuation of tissue damage.

Original languageEnglish
Pages (from-to)826-831
Number of pages6
JournalJournal of Rheumatology
Volume29
Issue number4
Publication statusPublished - 2002

Keywords

  • Juvenile idiopathic arthritis
  • Matrix metalloproteinases

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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