Serum Bile Acids Profiling in Inflammatory Bowel Disease Patients Treated with Anti-TNFs

Giulia Roda, Emanuele Porru, Konstantinos Katsanos, Alexandros Skamnelos, Kallirroi Kyriakidi, Gionata Fiorino, Dimitrios Christodoulou, Silvio Danese, Aldo Roda

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Inflammatory bowel diseases (IBD), ulcerative colitis (UC), and Crohn's disease (CD), represent systematic chronic conditions with a deficient intestinal absorption. We first attempt to investigate the serum bile acids (sBAs) profile in a large cohort of IBD patients to evaluate changes under anti-TNF alpha treatment.

METHODS: Forty CD and 40 UC patients were enrolled and BAs were quantified by high-pressure liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ES-MS/MS). Up to 15 different sBAs concentrations and clinical biomarkers where added to a Principal Component Analysis (PCA) to discriminate IBD from healthy conditions and treatment.

RESULTS: PCA allowed a separation into two clusters within CD (biologic-free patients and patients treated with anti-TNF alpha drugs and healthy subjects) but not UC. The first included CD. CD patients receiving anti-TNF alpha have an increase in total sBAs (4.11 1.23 μM) compared to patients not exposed. Secondary BAs significantly increase after anti-TNF alpha treatment (1.54 0.83 μM). Furthermore, multivariate analysis based on sBA concentration highlighted a different qualitative sBAs profile for UC and CD patients treated with conventional therapy.

CONCLUSION: According to our results, anti-TNF alpha in CD restores the sBA profile by re-establishing the physiological levels. These findings indicate that, secondary BAs might serve as an indirect biomarker of the healing process.

Original languageEnglish
JournalCells
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 2 2019

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