Serum concentrations of the interferon-γ-inducible chemokine IP-10/CXCL10 are augmented in both newly diagnosed type I diabetes mellitus patients and subjects at risk of developing the disease

F. Nicoletti, I. Conget, M. Di Mauro, R. Di Marco, M. Mazzarino, K. Bendtzen, A. Messina, R. Gomis

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Aims/hypothesis. Chemokines are chemotactic cytokines controlling the recruitment of leukocytes from the blood by regulating integrin adhesiveness. It has been shown that the migration of CD4+Th1 and CD4+Th2 cells is governed by specific chemokines. Increasing evidence suggests that the CD4+Th1 cheomoattractant chemokine CXCL10, also termed Interferon (IFN)-γ inducible protein (IP)-10 is pathogenetically involved in several immunoinflammatory and autoimmune diseases. Methods. IFN-γ and IP-10 were quantified by solid-phase ELISA in sera of patients with either newly diagnosed or long-term Type I (insulin-dependent) diabetes mellitus, and in sera of their healthy first degree relatives. The latter were subdivided into "low" and "high" risk prediabetic subjects depending on whether they were negative or positive for the anti-beta-cell autoantibodies ICA and GAD. Results. Compared with healthy control subjects (18%, 9/50), those with a low risk of disease (21%, 5/24) and the group of patients with long-term Type I diabetes (24%, 12/50), IP-10 was found more frequently and at increased concentrations in both newly diagnosed Type I diabetic patients (84%, 42/50) and in those with a high risk of disease (73%, 16/22); in the latter, the IP-10 concentrations correlated with those of IFN-γ. Conclusion/interpretation. Circulating IP-10 concentrations is increased in patients with Type I diabetes, but only during the early and subclinical stage of the disease.

Original languageEnglish
Pages (from-to)1107-1110
Number of pages4
JournalDiabetologia
Volume45
Issue number8
DOIs
Publication statusPublished - 2002

Fingerprint

Chemokine CXCL10
Type 1 Diabetes Mellitus
Chemokines
Interferons
Serum
Proteins
Adhesiveness
Th2 Cells
Integrins
Autoantibodies
Autoimmune Diseases
Healthy Volunteers
Leukocytes
Enzyme-Linked Immunosorbent Assay

Keywords

  • Autoimmunity
  • Chemokines
  • Cytokines
  • Interferon-gamma
  • IP-10/CXCL10

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Serum concentrations of the interferon-γ-inducible chemokine IP-10/CXCL10 are augmented in both newly diagnosed type I diabetes mellitus patients and subjects at risk of developing the disease. / Nicoletti, F.; Conget, I.; Di Mauro, M.; Di Marco, R.; Mazzarino, M.; Bendtzen, K.; Messina, A.; Gomis, R.

In: Diabetologia, Vol. 45, No. 8, 2002, p. 1107-1110.

Research output: Contribution to journalArticle

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abstract = "Aims/hypothesis. Chemokines are chemotactic cytokines controlling the recruitment of leukocytes from the blood by regulating integrin adhesiveness. It has been shown that the migration of CD4+Th1 and CD4+Th2 cells is governed by specific chemokines. Increasing evidence suggests that the CD4+Th1 cheomoattractant chemokine CXCL10, also termed Interferon (IFN)-γ inducible protein (IP)-10 is pathogenetically involved in several immunoinflammatory and autoimmune diseases. Methods. IFN-γ and IP-10 were quantified by solid-phase ELISA in sera of patients with either newly diagnosed or long-term Type I (insulin-dependent) diabetes mellitus, and in sera of their healthy first degree relatives. The latter were subdivided into {"}low{"} and {"}high{"} risk prediabetic subjects depending on whether they were negative or positive for the anti-beta-cell autoantibodies ICA and GAD. Results. Compared with healthy control subjects (18{\%}, 9/50), those with a low risk of disease (21{\%}, 5/24) and the group of patients with long-term Type I diabetes (24{\%}, 12/50), IP-10 was found more frequently and at increased concentrations in both newly diagnosed Type I diabetic patients (84{\%}, 42/50) and in those with a high risk of disease (73{\%}, 16/22); in the latter, the IP-10 concentrations correlated with those of IFN-γ. Conclusion/interpretation. Circulating IP-10 concentrations is increased in patients with Type I diabetes, but only during the early and subclinical stage of the disease.",
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AU - Nicoletti, F.

AU - Conget, I.

AU - Di Mauro, M.

AU - Di Marco, R.

AU - Mazzarino, M.

AU - Bendtzen, K.

AU - Messina, A.

AU - Gomis, R.

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N2 - Aims/hypothesis. Chemokines are chemotactic cytokines controlling the recruitment of leukocytes from the blood by regulating integrin adhesiveness. It has been shown that the migration of CD4+Th1 and CD4+Th2 cells is governed by specific chemokines. Increasing evidence suggests that the CD4+Th1 cheomoattractant chemokine CXCL10, also termed Interferon (IFN)-γ inducible protein (IP)-10 is pathogenetically involved in several immunoinflammatory and autoimmune diseases. Methods. IFN-γ and IP-10 were quantified by solid-phase ELISA in sera of patients with either newly diagnosed or long-term Type I (insulin-dependent) diabetes mellitus, and in sera of their healthy first degree relatives. The latter were subdivided into "low" and "high" risk prediabetic subjects depending on whether they were negative or positive for the anti-beta-cell autoantibodies ICA and GAD. Results. Compared with healthy control subjects (18%, 9/50), those with a low risk of disease (21%, 5/24) and the group of patients with long-term Type I diabetes (24%, 12/50), IP-10 was found more frequently and at increased concentrations in both newly diagnosed Type I diabetic patients (84%, 42/50) and in those with a high risk of disease (73%, 16/22); in the latter, the IP-10 concentrations correlated with those of IFN-γ. Conclusion/interpretation. Circulating IP-10 concentrations is increased in patients with Type I diabetes, but only during the early and subclinical stage of the disease.

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