TY - JOUR
T1 - Serum HBV-DNA in anti-HBe positive patients detected by filter and liquid phase hybridization assays
AU - Pontisso, P.
AU - Ruvoletto, M. G.
AU - Fattovich, G.
AU - Chemello, L.
AU - Morsica, G.
AU - Brollo, L.
AU - Matteotti, V.
AU - Alberti, A.
PY - 1989
Y1 - 1989
N2 - Serum HBV-DNA is considered the best parameter for monitoring HBV replication in the liver. The filter hybridization assay (spot test) with 32P-labelled HBV-DNA has been the technique more frequently used to date. A simple solution hybridization assay, in which 125I HBV-DNA is used as labelled probe, has been recently standardized. We have compared the performances of these two assays for the detection of HBV-DNA. The results were similar with the two methods: an agreement was found in 39 44 (89%) samples. Three sera were positive only by the spot assay and two only by the liquid phase assay. However, in these cases, HBV-DNA levels were near the sensitivity limits of the assay. Therefore, the filter and the liquid phase assays can be considered to be suitable methods to monitor HBV replication, a fundamental index for the clinical assessment and prognosis of patients with HBsAg positive chronic hepatitis.
AB - Serum HBV-DNA is considered the best parameter for monitoring HBV replication in the liver. The filter hybridization assay (spot test) with 32P-labelled HBV-DNA has been the technique more frequently used to date. A simple solution hybridization assay, in which 125I HBV-DNA is used as labelled probe, has been recently standardized. We have compared the performances of these two assays for the detection of HBV-DNA. The results were similar with the two methods: an agreement was found in 39 44 (89%) samples. Three sera were positive only by the spot assay and two only by the liquid phase assay. However, in these cases, HBV-DNA levels were near the sensitivity limits of the assay. Therefore, the filter and the liquid phase assays can be considered to be suitable methods to monitor HBV replication, a fundamental index for the clinical assessment and prognosis of patients with HBsAg positive chronic hepatitis.
KW - chronic liver disease
KW - molecular hybridization
KW - serum HBV-DNA
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U2 - 10.1016/0890-8508(89)90005-4
DO - 10.1016/0890-8508(89)90005-4
M3 - Article
C2 - 2677682
AN - SCOPUS:0024726090
VL - 3
SP - 245
EP - 249
JO - Molecular and Cellular Probes
JF - Molecular and Cellular Probes
SN - 0890-8508
IS - 3
ER -