Serum HBV-DNA in anti-HBe positive patients detected by filter and liquid phase hybridization assays

P. Pontisso, M. G. Ruvoletto, G. Fattovich, L. Chemello, G. Morsica, L. Brollo, V. Matteotti, A. Alberti

Research output: Contribution to journalArticlepeer-review

Abstract

Serum HBV-DNA is considered the best parameter for monitoring HBV replication in the liver. The filter hybridization assay (spot test) with 32P-labelled HBV-DNA has been the technique more frequently used to date. A simple solution hybridization assay, in which 125I HBV-DNA is used as labelled probe, has been recently standardized. We have compared the performances of these two assays for the detection of HBV-DNA. The results were similar with the two methods: an agreement was found in 39 44 (89%) samples. Three sera were positive only by the spot assay and two only by the liquid phase assay. However, in these cases, HBV-DNA levels were near the sensitivity limits of the assay. Therefore, the filter and the liquid phase assays can be considered to be suitable methods to monitor HBV replication, a fundamental index for the clinical assessment and prognosis of patients with HBsAg positive chronic hepatitis.

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalMolecular and Cellular Probes
Volume3
Issue number3
DOIs
Publication statusPublished - 1989

Keywords

  • chronic liver disease
  • molecular hybridization
  • serum HBV-DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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