Serum IgG antibodies to a 35-kDa P0-related glycoprotein in motor neuron disease

Eduardo Nobile-Orazio, Emanuela Manfredini, Manlio Sgarzi, Giorgio Spagnol, Silvia Allaria, Manfredo Quadroni, Guglielmo Scarlato

Research output: Contribution to journalArticle

Abstract

Using an immunoblot technique we found a significantly higher frequence of serum IgG antibodies to a 35-kDa peripheral nerve myelin glycoprotein in patients with motor neuron disease (MND) (39% of 70) than in patients with neuropathy (13% of 61), other neurological diseases (9% of 32) and normal subjects (5% of 20) (P <0.005 in all cases), but not with multiple sclerosis (MS) (20% of 30) or non-neural immune diseases (25% of 32). Most positive patients had antibody titers of 1:200 or 1:2000 while higher titers were only found in seven patients with MND, one with chronic inflammatory demyelinating neurophaty, two with MS, two with non-neural immune diseases and one with stroke. The reacting protein had a higher molecular mass than P0 and was only family bound by an anti-P0 antiserum, but had the same N-terminal amino acid sequence of P0. The difference in molecular mass between P0 and the 35-kDa protein and the IgG reactivity of one patient's IgG witht the 35-kDa protein persisted after its deglycosylation and dephosphorylation. Although there is no evidence that these antibodies are pathogenic, their frequent occurrence in MND and other immune-mediated conditions supports the hypothesis of an activation of the immune system in MND.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalJournal of Neuroimmunology
Volume53
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • Autoantibodies
  • Immunoglobulin G
  • Motor neuron disease
  • Myelin
  • P0 protein
  • Peripheral nerve

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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    Nobile-Orazio, E., Manfredini, E., Sgarzi, M., Spagnol, G., Allaria, S., Quadroni, M., & Scarlato, G. (1994). Serum IgG antibodies to a 35-kDa P0-related glycoprotein in motor neuron disease. Journal of Neuroimmunology, 53(2), 143-151. https://doi.org/10.1016/0165-5728(94)90024-8