Abstract
Allergic sensitization is mediated by immunoglobulin E (IgE) and an increase of their total value is frequently used to complete a correct diagnosis of atopy. Serum IgE may be considered a typical biomarker for the allergic phenotype. The aim of this study was to evaluate total serum IgE, according to sensitizations, and to find a cut off to discriminate between atopic and non-atopic subjects. Seven hundred and ninety-five patients were enrolled in this study. Serum levels of total IgE were measured by a fluorescence immunoassay (ImmunoCAP; ThermoFisher, Uppsala, Sweden) while specific IgE levels were measured by immunofluorometric assay (ImmunoCAP; ThermoFisher, Uppsala, Sweden). Both tests were expressed in kU/L, according to manufacturer's instructions. Results: A difference for total IgE, according to the gender, has been found (p = 0, 0012 ) with higher values for males than for females. A correlation has been found between total IgE and specific IgE, even distinguishing the population in sensitized and non-sensitized. A statistically significant difference has been found according to the presence or the absence of sensitization (p < 0, 0001) and also considering mono-sensitized and poly-sensitized patients (p < 0, 0001). ROC analysis has been performed to define a cut off for total serum IgE, according to sensitization and to the type of sensitization (mono-sensitization or poly-sensitization). Finally multiple regression models have been performed to describe total IgE response (positive or negative) and to predict total IgE values. Since clinical limitations are well known, total IgE provide a useful aid to define atopy, allowing the clinician to carry out further investigations in patients with total IgE values beyond normal limits.
| Original language | English |
|---|---|
| Pages (from-to) | 64-69 |
| Number of pages | 6 |
| Journal | Journal of Biological Regulators and Homeostatic Agents |
| Volume | 29 |
| Issue number | 2 |
| Publication status | Published - 2015 |
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Keywords
- Sensitization
- Specific IgE
- Total IgE
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Immunology and Allergy
- Physiology
- Immunology
- Medicine(all)
- Oncology
- Endocrinology
- Physiology (medical)
- Cancer Research
Cite this
Serum immunoglobulin IgE in a pediatric population : A retrospective analysis. / De Amici, M.; Marseglia, A.; Licari, A.; Caimmi, S.; Ricci, A.; Nigrisoli, S.; Torre, C.; Testa, G.; Marseglia, G. L.
In: Journal of Biological Regulators and Homeostatic Agents, Vol. 29, No. 2, 2015, p. 64-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serum immunoglobulin IgE in a pediatric population
T2 - A retrospective analysis
AU - De Amici, M.
AU - Marseglia, A.
AU - Licari, A.
AU - Caimmi, S.
AU - Ricci, A.
AU - Nigrisoli, S.
AU - Torre, C.
AU - Testa, G.
AU - Marseglia, G. L.
PY - 2015
Y1 - 2015
N2 - Allergic sensitization is mediated by immunoglobulin E (IgE) and an increase of their total value is frequently used to complete a correct diagnosis of atopy. Serum IgE may be considered a typical biomarker for the allergic phenotype. The aim of this study was to evaluate total serum IgE, according to sensitizations, and to find a cut off to discriminate between atopic and non-atopic subjects. Seven hundred and ninety-five patients were enrolled in this study. Serum levels of total IgE were measured by a fluorescence immunoassay (ImmunoCAP; ThermoFisher, Uppsala, Sweden) while specific IgE levels were measured by immunofluorometric assay (ImmunoCAP; ThermoFisher, Uppsala, Sweden). Both tests were expressed in kU/L, according to manufacturer's instructions. Results: A difference for total IgE, according to the gender, has been found (p = 0, 0012 ) with higher values for males than for females. A correlation has been found between total IgE and specific IgE, even distinguishing the population in sensitized and non-sensitized. A statistically significant difference has been found according to the presence or the absence of sensitization (p < 0, 0001) and also considering mono-sensitized and poly-sensitized patients (p < 0, 0001). ROC analysis has been performed to define a cut off for total serum IgE, according to sensitization and to the type of sensitization (mono-sensitization or poly-sensitization). Finally multiple regression models have been performed to describe total IgE response (positive or negative) and to predict total IgE values. Since clinical limitations are well known, total IgE provide a useful aid to define atopy, allowing the clinician to carry out further investigations in patients with total IgE values beyond normal limits.
AB - Allergic sensitization is mediated by immunoglobulin E (IgE) and an increase of their total value is frequently used to complete a correct diagnosis of atopy. Serum IgE may be considered a typical biomarker for the allergic phenotype. The aim of this study was to evaluate total serum IgE, according to sensitizations, and to find a cut off to discriminate between atopic and non-atopic subjects. Seven hundred and ninety-five patients were enrolled in this study. Serum levels of total IgE were measured by a fluorescence immunoassay (ImmunoCAP; ThermoFisher, Uppsala, Sweden) while specific IgE levels were measured by immunofluorometric assay (ImmunoCAP; ThermoFisher, Uppsala, Sweden). Both tests were expressed in kU/L, according to manufacturer's instructions. Results: A difference for total IgE, according to the gender, has been found (p = 0, 0012 ) with higher values for males than for females. A correlation has been found between total IgE and specific IgE, even distinguishing the population in sensitized and non-sensitized. A statistically significant difference has been found according to the presence or the absence of sensitization (p < 0, 0001) and also considering mono-sensitized and poly-sensitized patients (p < 0, 0001). ROC analysis has been performed to define a cut off for total serum IgE, according to sensitization and to the type of sensitization (mono-sensitization or poly-sensitization). Finally multiple regression models have been performed to describe total IgE response (positive or negative) and to predict total IgE values. Since clinical limitations are well known, total IgE provide a useful aid to define atopy, allowing the clinician to carry out further investigations in patients with total IgE values beyond normal limits.
KW - Sensitization
KW - Specific IgE
KW - Total IgE
UR - http://www.scopus.com/inward/record.url?scp=84986220622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986220622&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84986220622
VL - 29
SP - 64
EP - 69
JO - Journal of Biological Regulators and Homeostatic Agents
JF - Journal of Biological Regulators and Homeostatic Agents
SN - 0393-974X
IS - 2
ER -