Serum immunoglobulin IgE in a pediatric population: A retrospective analysis

M. De Amici, A. Marseglia, A. Licari, S. Caimmi, A. Ricci, S. Nigrisoli, C. Torre, G. Testa, G. L. Marseglia

Research output: Contribution to journalArticlepeer-review


Allergic sensitization is mediated by immunoglobulin E (IgE) and an increase of their total value is frequently used to complete a correct diagnosis of atopy. Serum IgE may be considered a typical biomarker for the allergic phenotype. The aim of this study was to evaluate total serum IgE, according to sensitizations, and to find a cut off to discriminate between atopic and non-atopic subjects. Seven hundred and ninety-five patients were enrolled in this study. Serum levels of total IgE were measured by a fluorescence immunoassay (ImmunoCAP; ThermoFisher, Uppsala, Sweden) while specific IgE levels were measured by immunofluorometric assay (ImmunoCAP; ThermoFisher, Uppsala, Sweden). Both tests were expressed in kU/L, according to manufacturer's instructions. Results: A difference for total IgE, according to the gender, has been found (p = 0, 0012 ) with higher values for males than for females. A correlation has been found between total IgE and specific IgE, even distinguishing the population in sensitized and non-sensitized. A statistically significant difference has been found according to the presence or the absence of sensitization (p < 0, 0001) and also considering mono-sensitized and poly-sensitized patients (p < 0, 0001). ROC analysis has been performed to define a cut off for total serum IgE, according to sensitization and to the type of sensitization (mono-sensitization or poly-sensitization). Finally multiple regression models have been performed to describe total IgE response (positive or negative) and to predict total IgE values. Since clinical limitations are well known, total IgE provide a useful aid to define atopy, allowing the clinician to carry out further investigations in patients with total IgE values beyond normal limits.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalJournal of Biological Regulators and Homeostatic Agents
Issue number2
Publication statusPublished - 2015


  • Sensitization
  • Specific IgE
  • Total IgE

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy
  • Physiology
  • Immunology
  • Medicine(all)
  • Oncology
  • Endocrinology
  • Physiology (medical)
  • Cancer Research


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