TY - JOUR
T1 - Serum iron and matrix metalloproteinase-9 variations in limbs affected by chronic venous disease and venous leg ulcers
AU - Zamboni, Paolo
AU - Scapoli, Gianluigi
AU - Lanzara, Vincenzo
AU - Izzo, Marcello
AU - Fortini, Patrizia
AU - Legnaro, Andrea
AU - Palazzo, Annunziata
AU - Tognazzo, Silvia
AU - Gemmati, Donato
PY - 2005
Y1 - 2005
N2 - BACKGROUND. Severe chronic venous disease (CVD) is characterized by both dermal hemosiderin accumulation and matrix metalloproteinase (MMP) hyperactivation. The iron-driven pathway is one of the recognized mechanisms of MMP hyperactivation. OBJECTIVE. To investigate the potential consequences of leg hemosiderin deposits on both iron metabolism and activation of MMPs. METHODS. We contemporaneously assessed the following in the serum of the arm and ankle veins of 30 patients (C4-6) with CVD and 14 normal subjects: ferritin, transferrin, iron, percentage of transferrin iron binding capacity (%TIBC), and MMP-9. Optical microscopy examinations with Perls' staining of chronic wounds were also performed. RESULTS. Histology consistently revealed iron deposits. Serum ferritin, iron, and %TIBC were significantly increased in the legs affected by severe CVD compared with the arm of the same subjects or the controls. In addition, iron and %TIBC were significantly elevated in the legs of ulcer patients. The rate of activation of MMP-9 was significantly elevated in CVD. CONCLUSIONS. The increased iron deposition in legs affected by CVD seems to be more instable in ulcer patients, leading to iron release in the serum of the affected leg. Our data suggest the iron-driven pathway as a further mechanism for MMP hyperexpression leading to tissue lesion.
AB - BACKGROUND. Severe chronic venous disease (CVD) is characterized by both dermal hemosiderin accumulation and matrix metalloproteinase (MMP) hyperactivation. The iron-driven pathway is one of the recognized mechanisms of MMP hyperactivation. OBJECTIVE. To investigate the potential consequences of leg hemosiderin deposits on both iron metabolism and activation of MMPs. METHODS. We contemporaneously assessed the following in the serum of the arm and ankle veins of 30 patients (C4-6) with CVD and 14 normal subjects: ferritin, transferrin, iron, percentage of transferrin iron binding capacity (%TIBC), and MMP-9. Optical microscopy examinations with Perls' staining of chronic wounds were also performed. RESULTS. Histology consistently revealed iron deposits. Serum ferritin, iron, and %TIBC were significantly increased in the legs affected by severe CVD compared with the arm of the same subjects or the controls. In addition, iron and %TIBC were significantly elevated in the legs of ulcer patients. The rate of activation of MMP-9 was significantly elevated in CVD. CONCLUSIONS. The increased iron deposition in legs affected by CVD seems to be more instable in ulcer patients, leading to iron release in the serum of the affected leg. Our data suggest the iron-driven pathway as a further mechanism for MMP hyperexpression leading to tissue lesion.
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M3 - Article
C2 - 15996413
AN - SCOPUS:22844447869
VL - 31
SP - 644
EP - 649
JO - Dermatologic Surgery
JF - Dermatologic Surgery
SN - 1076-0512
IS - 6
ER -