Serum levels of the hepcidin-20 isoform in a large general population: The Val Borbera study

Natascia Campostrini, Michela Traglia, Nicola Martinelli, Michela Corbella, Massimiliano Cocca, Daniele Manna, Annalisa Castagna, Corrado Masciullo, Laura Silvestri, Oliviero Olivieri, Daniela Toniolo, Clara Camaschella, Domenico Girelli

Research output: Contribution to journalArticle

Abstract

Hepcidin, a 25 amino-acid liver hormone, has recently emerged as the key regulator of iron homeostasis. Proteomic studies in limited number of subjects have shown that biological fluids can also contain truncated isoforms, whose role remains to be elucidated. We report, for the first time, data about serum levels of the hepcidin-20 isoform (hep-20) in a general population, taking advantage of the Val Borbera (VB) study where hepcidin-25 (hep-25) was measured by SELDI-TOF-MS. Detectable amount of hep-20 were found in sera from 854 out of 1577 subjects (54.2%), and its levels were about 14% of hep-25 levels. A small fraction of subjects (n = 30, 1.9%) had detectable hep-20 but undetectable hep-25. In multivariate regression models, significant predictors of hep-20 were hep-25 and age in males, and hep-25, age, serum ferritin and body mass index in females. Of note, the hep-25:hep-20 ratio was not constant in the VB population, but increased progressively with increasing ferritin levels. This is not consistent with the simplistic view of hep-20 as a mere catabolic byproduct of hep-25. Although a possible active regulation of hep-20 production needs further confirmation, our results may also have implications for immunoassays for serum hepcidin based on antibodies lacking specificity for hep-25.This article is part of a Special Issue entitled: Integrated omics.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalJournal of Proteomics
Volume76
DOIs
Publication statusPublished - Dec 5 2012

Keywords

  • Ferritin
  • Hepcidin
  • Iron metabolism
  • SELDI-TOF-MS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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    Campostrini, N., Traglia, M., Martinelli, N., Corbella, M., Cocca, M., Manna, D., Castagna, A., Masciullo, C., Silvestri, L., Olivieri, O., Toniolo, D., Camaschella, C., & Girelli, D. (2012). Serum levels of the hepcidin-20 isoform in a large general population: The Val Borbera study. Journal of Proteomics, 76, 28-35. https://doi.org/10.1016/j.jprot.2012.08.006