Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression

An immunoenzymatic study from the Italian group of immunopathology

Pietro Quaglino, Marzia Caproni, Emiliano Antiga, Elena Del Bianco, Simona Osella-Abate, Paola Savoia, Alessandra Frezzolini, Donatella Schena, Angelo Marzano, Walter Volpi, Clara De Simone, Aurora Parodi, Paolo Fabbri, Maria Grazia Bernengo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalDermatology
Volume214
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Chemokine CCL17
Erythema Multiforme
Stevens-Johnson Syndrome
Fas Ligand Protein
Interleukin-12
Chemokines
Chemokine CCL22
Serum
Tissue Donors
T-Lymphocytes
Up-Regulation
Enzyme-Linked Immunosorbent Assay

Keywords

  • Erythema multiforme
  • Stevens-Johnson syndrome/toxic epidermal necrolysis
  • Thymus- and activation-regulated chemokine

ASJC Scopus subject areas

  • Dermatology

Cite this

Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression : An immunoenzymatic study from the Italian group of immunopathology. / Quaglino, Pietro; Caproni, Marzia; Antiga, Emiliano; Del Bianco, Elena; Osella-Abate, Simona; Savoia, Paola; Frezzolini, Alessandra; Schena, Donatella; Marzano, Angelo; Volpi, Walter; De Simone, Clara; Parodi, Aurora; Fabbri, Paolo; Bernengo, Maria Grazia.

In: Dermatology, Vol. 214, No. 4, 04.2007, p. 296-304.

Research output: Contribution to journalArticle

Quaglino, Pietro ; Caproni, Marzia ; Antiga, Emiliano ; Del Bianco, Elena ; Osella-Abate, Simona ; Savoia, Paola ; Frezzolini, Alessandra ; Schena, Donatella ; Marzano, Angelo ; Volpi, Walter ; De Simone, Clara ; Parodi, Aurora ; Fabbri, Paolo ; Bernengo, Maria Grazia. / Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression : An immunoenzymatic study from the Italian group of immunopathology. In: Dermatology. 2007 ; Vol. 214, No. 4. pp. 296-304.
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abstract = "Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.",
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T1 - Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression

T2 - An immunoenzymatic study from the Italian group of immunopathology

AU - Quaglino, Pietro

AU - Caproni, Marzia

AU - Antiga, Emiliano

AU - Del Bianco, Elena

AU - Osella-Abate, Simona

AU - Savoia, Paola

AU - Frezzolini, Alessandra

AU - Schena, Donatella

AU - Marzano, Angelo

AU - Volpi, Walter

AU - De Simone, Clara

AU - Parodi, Aurora

AU - Fabbri, Paolo

AU - Bernengo, Maria Grazia

PY - 2007/4

Y1 - 2007/4

N2 - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

AB - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

KW - Erythema multiforme

KW - Stevens-Johnson syndrome/toxic epidermal necrolysis

KW - Thymus- and activation-regulated chemokine

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