Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke

Federico Carbone, Nicolas Vuilleumier, Fabienne Burger, Gloria Roversi, Carmine Tamborino, Ilaria Casetta, Silva Seraceni, Alessandro Trentini, Marina Padroni, Franco Dallegri, François Mach, Enrico Fainardi, Fabrizio Montecucco

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: After an acute ischaemic stroke (AIS), several inflammatory biomarkers have been investigated, but their predictive role on functional recovery remains to be validated. Here, we investigated the prognostic relevance of biomarkers related to atherosclerotic plaque calcification, such as osteopontin (OPN), osteoprotegerin (OPG) and the receptor activator of nuclear factor kappa-B ligand (RANKL) in a cohort of patients with AIS (n = 90) during 90-day follow-up. Materials and methods: Radiological and clinical examinations as well as blood sampling were performed at admission and at days 1, 7 and 90 from the event. Validated scores [such as modified Rankin scale (mRS) and the National Institutes of Health Stroke Scale (NIHSS)] were used to assess poststroke outcome. Serum levels of OPN, OPG and RANKL were measured by colorimetric enzyme-linked immunosorbent assay (ELISA). Results: When compared to the admission, OPN serum levels increased at day 7. Serum OPN levels at this time point were positively correlated with both ischaemic lesion volume and NIHSS at days 7 and 90. A cut-off of 30·53 ng/mL was identified for serum OPN by receiver operating characteristic (ROC) curve analysis. Adjusted logistic regression showed that serum OPN levels at day 7 predicted worse mRS at day 90 [OR 4·13 (95% CI 1·64-10·36); P = 0·002] and NIHSS [1·49 (95% CI 1·16-1·99); P = 0·007], independently of age, gender, hypertension and thrombolysis. Conclusions: Serum levels of OPN, but not OPG and RANKL, peaked at day 7 after AIS and predicted worse neurological scores. Therefore, OPN might have a pathophysiological and clinical relevance after AIS.

Original languageEnglish
Pages (from-to)579-586
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume45
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Osteopontin
Stroke
RANK Ligand
Serum
Osteoprotegerin
National Institutes of Health (U.S.)
Health
Biomarkers
Calcification (biochemistry)
Immunosorbents
Atherosclerotic Plaques
ROC Curve
Logistics
Assays
Blood
Logistic Models
Enzyme-Linked Immunosorbent Assay
Sampling
Hypertension
Recovery

Keywords

  • Ischaemic stroke
  • Osteopontin
  • Osteoprotegerin
  • Receptor activator of nuclear factor kappa-B ligand

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Carbone, F., Vuilleumier, N., Burger, F., Roversi, G., Tamborino, C., Casetta, I., ... Montecucco, F. (2015). Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke. European Journal of Clinical Investigation, 45(6), 579-586. https://doi.org/10.1111/eci.12446

Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke. / Carbone, Federico; Vuilleumier, Nicolas; Burger, Fabienne; Roversi, Gloria; Tamborino, Carmine; Casetta, Ilaria; Seraceni, Silva; Trentini, Alessandro; Padroni, Marina; Dallegri, Franco; Mach, François; Fainardi, Enrico; Montecucco, Fabrizio.

In: European Journal of Clinical Investigation, Vol. 45, No. 6, 01.06.2015, p. 579-586.

Research output: Contribution to journalArticle

Carbone, F, Vuilleumier, N, Burger, F, Roversi, G, Tamborino, C, Casetta, I, Seraceni, S, Trentini, A, Padroni, M, Dallegri, F, Mach, F, Fainardi, E & Montecucco, F 2015, 'Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke', European Journal of Clinical Investigation, vol. 45, no. 6, pp. 579-586. https://doi.org/10.1111/eci.12446
Carbone, Federico ; Vuilleumier, Nicolas ; Burger, Fabienne ; Roversi, Gloria ; Tamborino, Carmine ; Casetta, Ilaria ; Seraceni, Silva ; Trentini, Alessandro ; Padroni, Marina ; Dallegri, Franco ; Mach, François ; Fainardi, Enrico ; Montecucco, Fabrizio. / Serum osteopontin levels are upregulated and predict disability after an ischaemic stroke. In: European Journal of Clinical Investigation. 2015 ; Vol. 45, No. 6. pp. 579-586.
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abstract = "Background: After an acute ischaemic stroke (AIS), several inflammatory biomarkers have been investigated, but their predictive role on functional recovery remains to be validated. Here, we investigated the prognostic relevance of biomarkers related to atherosclerotic plaque calcification, such as osteopontin (OPN), osteoprotegerin (OPG) and the receptor activator of nuclear factor kappa-B ligand (RANKL) in a cohort of patients with AIS (n = 90) during 90-day follow-up. Materials and methods: Radiological and clinical examinations as well as blood sampling were performed at admission and at days 1, 7 and 90 from the event. Validated scores [such as modified Rankin scale (mRS) and the National Institutes of Health Stroke Scale (NIHSS)] were used to assess poststroke outcome. Serum levels of OPN, OPG and RANKL were measured by colorimetric enzyme-linked immunosorbent assay (ELISA). Results: When compared to the admission, OPN serum levels increased at day 7. Serum OPN levels at this time point were positively correlated with both ischaemic lesion volume and NIHSS at days 7 and 90. A cut-off of 30·53 ng/mL was identified for serum OPN by receiver operating characteristic (ROC) curve analysis. Adjusted logistic regression showed that serum OPN levels at day 7 predicted worse mRS at day 90 [OR 4·13 (95{\%} CI 1·64-10·36); P = 0·002] and NIHSS [1·49 (95{\%} CI 1·16-1·99); P = 0·007], independently of age, gender, hypertension and thrombolysis. Conclusions: Serum levels of OPN, but not OPG and RANKL, peaked at day 7 after AIS and predicted worse neurological scores. Therefore, OPN might have a pathophysiological and clinical relevance after AIS.",
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AU - Vuilleumier, Nicolas

AU - Burger, Fabienne

AU - Roversi, Gloria

AU - Tamborino, Carmine

AU - Casetta, Ilaria

AU - Seraceni, Silva

AU - Trentini, Alessandro

AU - Padroni, Marina

AU - Dallegri, Franco

AU - Mach, François

AU - Fainardi, Enrico

AU - Montecucco, Fabrizio

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N2 - Background: After an acute ischaemic stroke (AIS), several inflammatory biomarkers have been investigated, but their predictive role on functional recovery remains to be validated. Here, we investigated the prognostic relevance of biomarkers related to atherosclerotic plaque calcification, such as osteopontin (OPN), osteoprotegerin (OPG) and the receptor activator of nuclear factor kappa-B ligand (RANKL) in a cohort of patients with AIS (n = 90) during 90-day follow-up. Materials and methods: Radiological and clinical examinations as well as blood sampling were performed at admission and at days 1, 7 and 90 from the event. Validated scores [such as modified Rankin scale (mRS) and the National Institutes of Health Stroke Scale (NIHSS)] were used to assess poststroke outcome. Serum levels of OPN, OPG and RANKL were measured by colorimetric enzyme-linked immunosorbent assay (ELISA). Results: When compared to the admission, OPN serum levels increased at day 7. Serum OPN levels at this time point were positively correlated with both ischaemic lesion volume and NIHSS at days 7 and 90. A cut-off of 30·53 ng/mL was identified for serum OPN by receiver operating characteristic (ROC) curve analysis. Adjusted logistic regression showed that serum OPN levels at day 7 predicted worse mRS at day 90 [OR 4·13 (95% CI 1·64-10·36); P = 0·002] and NIHSS [1·49 (95% CI 1·16-1·99); P = 0·007], independently of age, gender, hypertension and thrombolysis. Conclusions: Serum levels of OPN, but not OPG and RANKL, peaked at day 7 after AIS and predicted worse neurological scores. Therefore, OPN might have a pathophysiological and clinical relevance after AIS.

AB - Background: After an acute ischaemic stroke (AIS), several inflammatory biomarkers have been investigated, but their predictive role on functional recovery remains to be validated. Here, we investigated the prognostic relevance of biomarkers related to atherosclerotic plaque calcification, such as osteopontin (OPN), osteoprotegerin (OPG) and the receptor activator of nuclear factor kappa-B ligand (RANKL) in a cohort of patients with AIS (n = 90) during 90-day follow-up. Materials and methods: Radiological and clinical examinations as well as blood sampling were performed at admission and at days 1, 7 and 90 from the event. Validated scores [such as modified Rankin scale (mRS) and the National Institutes of Health Stroke Scale (NIHSS)] were used to assess poststroke outcome. Serum levels of OPN, OPG and RANKL were measured by colorimetric enzyme-linked immunosorbent assay (ELISA). Results: When compared to the admission, OPN serum levels increased at day 7. Serum OPN levels at this time point were positively correlated with both ischaemic lesion volume and NIHSS at days 7 and 90. A cut-off of 30·53 ng/mL was identified for serum OPN by receiver operating characteristic (ROC) curve analysis. Adjusted logistic regression showed that serum OPN levels at day 7 predicted worse mRS at day 90 [OR 4·13 (95% CI 1·64-10·36); P = 0·002] and NIHSS [1·49 (95% CI 1·16-1·99); P = 0·007], independently of age, gender, hypertension and thrombolysis. Conclusions: Serum levels of OPN, but not OPG and RANKL, peaked at day 7 after AIS and predicted worse neurological scores. Therefore, OPN might have a pathophysiological and clinical relevance after AIS.

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