Serum Soluble Interieukin 6 (IL-6) Receptor and IL-6/ Soluble IL-6 Receptor Complex in Systemic Juvenile Rheumatoid Arthritis

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Abstract

By using a sandwich ELISA, soluble human IL-6 receptor (sIL-6 R) levels were measured in the sera of 20 healthy children and of 25 patients with systemic juvenile rheumatoid arthritis (JRA). In patients with systemic JRA, serum sIL-6 R levels (114.6±37.7 ng/ml) were significantly lower (P <0.01) than those of healthy children (161.2±45.5 ng/ml). Serum sIL-6 R levels were negatively correlated (r = -0.610, P <0.001) with serum IL-6 levels measured with the B9 cells. The serum IL-6/sIL-6 R complex was detected using an ELISA based on a monoclonal antibody to IL-6 for capture and on a monoclonal antibody to human sIL-6 R for detection. Healthy controls had little, if any, detectable serum IL-6/sIL-6 R complex (OD 0.024±0.027), while the majority of patients with systemic JRA presented measurable serum IL-6/sIL-6 R complex (OD 0.492±0.546). IL-6 levels estimated in the circulating IL-6/SIL-6 R complexes were in the range of nanograms per milliliter and ∼ 20-fold higher than those measured by the B9 cells. Since serum C-reactive protein concentrations were much more correlated with serum levels of IL-6/sIL-6 R complexes (r = 0.713, r2 = 0.51, P <0.0001) than with the serum IL-6 levels measured with the B9 cells (r = 0.435, r2 = 0.19, P = 0.05), the large quantities of serum IL-6 present in IL-6/sIL-6 R complexes appear to be biologically relevant in vivo, at least as far as the induction by IL-6 of acute phase protein production.

Original languageEnglish
Pages (from-to)2114-2119
Number of pages6
JournalJournal of Clinical Investigation
Volume93
Issue number5
Publication statusPublished - May 1994

Keywords

  • C-reactive protein
  • Interleukin 6
  • Interleukin 6 receptor
  • Juvenile rheumatoid arthritis

ASJC Scopus subject areas

  • Medicine(all)

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