Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells.

Giuliana Gobbi, Luca Sangiorgi, Luca Lenzi, Raffaella Casadei, Silvia Canaider, Pierluigi Strippoli, Enrico Lucarelli, Ilaria Ghedini, Davide Donati, Nicola Fabbri, Jorg Warzecha, Choh Yeoung, Lee J. Helman, Piero Picci, Paolo Carinci

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Members of the bone morphogenetic protein (BMP) family and their receptors (BMPRs and activin receptors-ActRs) promote the development of bones with a fine regulation of their expression. Mutations in BMPs or BMPRs cause several diseases, as shown in knockout mice, such as skeletal defects, familial primary pulmonary hypertension and neoplasias. Osteosarcoma is the most frequent primary malignant tumor of bone. Due to their importance in bone development, BMPs, BMPRs and ActRs could also play a role in osteosarcoma growth and development. Previous data have shown that the overexpression of the BMPR-II was related to poor prognosis in malignant and metastatic bone tumors. We evaluate by reverse transcription-linked polymerase chain reaction analysis (RT-PCR) the expression pattern of BMPs, BMPRs and ActRs in five different human osteosarcoma cell lines (MG63, G292, HOS, SaOS and U2). Moreover, we performed the mutational screening of the complete BMPR-II mRNA by automated sequencing of the correspondent cDNA to evaluate the presence of point mutations in osteosarcoma cell lines. All the osteosarcoma cell lines studied simultaneously expressed the BMPs, BMPRs and ActRs investigated. No mutations were detected in the BMPR-II cDNA. Our results suggest the presence of a mechanism involving the simultaneous activation of the BMPs and their receptors in osteosarcoma cell lines.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalInternational Journal of Oncology
Volume20
Issue number1
Publication statusPublished - Jan 2002

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Osteosarcoma
Cell Line
Bone Development
Complementary DNA
Activin Receptors
Bone Morphogenetic Protein Receptors
Bone and Bones
Neoplasms
Mutation
Bone Morphogenetic Proteins
Growth and Development
Point Mutation
Knockout Mice
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Gobbi, G., Sangiorgi, L., Lenzi, L., Casadei, R., Canaider, S., Strippoli, P., ... Carinci, P. (2002). Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells. International Journal of Oncology, 20(1), 143-147.

Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells. / Gobbi, Giuliana; Sangiorgi, Luca; Lenzi, Luca; Casadei, Raffaella; Canaider, Silvia; Strippoli, Pierluigi; Lucarelli, Enrico; Ghedini, Ilaria; Donati, Davide; Fabbri, Nicola; Warzecha, Jorg; Yeoung, Choh; Helman, Lee J.; Picci, Piero; Carinci, Paolo.

In: International Journal of Oncology, Vol. 20, No. 1, 01.2002, p. 143-147.

Research output: Contribution to journalArticle

Gobbi, G, Sangiorgi, L, Lenzi, L, Casadei, R, Canaider, S, Strippoli, P, Lucarelli, E, Ghedini, I, Donati, D, Fabbri, N, Warzecha, J, Yeoung, C, Helman, LJ, Picci, P & Carinci, P 2002, 'Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells.', International Journal of Oncology, vol. 20, no. 1, pp. 143-147.
Gobbi, Giuliana ; Sangiorgi, Luca ; Lenzi, Luca ; Casadei, Raffaella ; Canaider, Silvia ; Strippoli, Pierluigi ; Lucarelli, Enrico ; Ghedini, Ilaria ; Donati, Davide ; Fabbri, Nicola ; Warzecha, Jorg ; Yeoung, Choh ; Helman, Lee J. ; Picci, Piero ; Carinci, Paolo. / Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells. In: International Journal of Oncology. 2002 ; Vol. 20, No. 1. pp. 143-147.
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