Several nuclear events during apoptosis depend on caspase-3 activation but do not constitute a common pathway

Lisa Trisciuoglio, Marco Emilio Bianchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A number of nuclear events occur during apoptosis, including DNA laddering, nuclear lamina breakdown, phosphorylation of histones H2B and histone H2AX, and the tight binding to chromatin of HMGB1 and CAD, the nuclease responsible for DNA laddering. We have performed an epistasis analysis to investigate whether these events cluster together in pathways. We find that all depend directly or indirectly on caspase-3 activation. CAD activation, H2AX phosphorylation and DNA laddering cluster together into a pathway, but all other events appear to be independent of each other downstream of caspase-3, and likely evolved subject to different functional pressures.

Original languageEnglish
Article numbere6234
JournalPLoS One
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 29 2009

Fingerprint

Phosphorylation
caspase-3
histones
Caspase 3
Histones
Computer aided design
phosphorylation
apoptosis
Chemical activation
Nuclear Lamina
Apoptosis
HMGB1 Protein
Deoxyribonucleases
nucleases
DNA
epistasis
nuclear genome
Chromatin
chromatin
Pressure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Several nuclear events during apoptosis depend on caspase-3 activation but do not constitute a common pathway. / Trisciuoglio, Lisa; Bianchi, Marco Emilio.

In: PLoS One, Vol. 4, No. 7, e6234, 29.07.2009.

Research output: Contribution to journalArticle

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